mortality/aging
• at E18.5, only 50% of the expected number of compound heterozygotes are found
|
growth/size/body
• embryos are categorized into 3 groups determined by severity of phenotype; embryos are generally smaller than wild-type littermates in the least severely affected group 1
• in group 2, embryos are very small and developmentally delayed compared to littermates but mesoderm-derived structures are present
• group 3 embryos which are the most severely affected group resemble Fgf8tm1.2Mrt homozygotes
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embryo
• many group 2 embryos display a failure of posterior development
|
• embryos are categorized into 3 groups determined by severity of phenotype; embryos are generally smaller than wild-type littermates in the least severely affected group 1
• in group 2, embryos are very small and developmentally delayed compared to littermates but mesoderm-derived structures are present
• group 3 embryos which are the most severely affected group resemble Fgf8tm1.2Mrt homozygotes
|
nervous system
• mutants exhibit severe abnormalities in the pituitary gland
• at E17.5, 2 of 6 mutants show a substantial reduction of anterior pituitary tissue and absence of the posterior lobe while 4 of 6 mutants show a mild pituitary gland phenotype
|
• vesicles are abnormally small in group 1 embryos
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• defects are usually more severe than in Fgftm1.1 homozygotes
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• inferior collicular (posterior midbrain) tissue is deleted in group 1 embryos
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• reduction of arginine vasopressin and oxytocin neurons in the supraoptic, suprachiasmatic, and paraventricular nuclei
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• cerebellar tissue (anterior hindbrain) is absent in group 1 embryos; defects are usually more severe than in Fgftm1.1 homozygotes
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cardiovascular system
• many group 2 embryos display cardiac abnormalities
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limbs/digits/tail
syndactyly
(
J:45909
)
• fusion of two digits is often observed
|
craniofacial
endocrine/exocrine glands
• mutants exhibit severe abnormalities in the pituitary gland
• at E17.5, 2 of 6 mutants show a substantial reduction of anterior pituitary tissue and absence of the posterior lobe while 4 of 6 mutants show a mild pituitary gland phenotype
|