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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Daam1tm1.1Tpy
targeted mutation 1.1, Terry Yamaguchi
MGI:6149780
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Daam1tm1.1Tpy/Daam1tm1.1Tpy
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Nkx2-5tm1(cre)Rjs/Nkx2-5+
involves: 129S1/Sv * 129S4/SvJaeSor * 129S7/SvEvBrd MGI:6150944
cn2
Daam1tm1.1Tpy/Daam1tm1.1Tpy
Nkx2-5tm1(cre)Rjs/Nkx2-5+
involves: 129S1/Sv * 129S7/SvEvBrd MGI:6150918
cn3
Daam1tm1.1Tpy/Daam1tm1.1Tpy
Daam2tm1Tpy/Daam2tm1Tpy
Nkx2-5tm1(cre)Rjs/Nkx2-5+
involves: 129S1/Sv * 129S7/SvEvBrd MGI:6150919
cn4
Daam1tm1.1Tpy/Daam1tm1.1Tpy
Nkx2-5tm1(cre)Rjs/Nkx2-5+
Wnt5atm1Amc/Wnt5atm1Amc
involves: 129S1/Sv * 129S7/SvEvBrd MGI:6150926
cn5
Daam1tm1.1Tpy/Daam1tm1.1Tpy
Nkx2-5tm1(cre)Rjs/Nkx2-5+
Wnt5atm1Amc/Wnt5a+
involves: 129S1/Sv * 129S7/SvEvBrd MGI:6151066


Genotype
MGI:6150944
cn1
Allelic
Composition
Daam1tm1.1Tpy/Daam1tm1.1Tpy
Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
Nkx2-5tm1(cre)Rjs/Nkx2-5+
Genetic
Background
involves: 129S1/Sv * 129S4/SvJaeSor * 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Daam1tm1.1Tpy mutation (0 available); any Daam1 mutation (21 available)
Gt(ROSA)26Sortm1Sor mutation (7 available); any Gt(ROSA)26Sor mutation (523 available)
Nkx2-5tm1(cre)Rjs mutation (1 available); any Nkx2-5 mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• in E13.5 embryos
• cardiomyocytes smaller, rounded and loosely attached to each other with thin cellular projections in conotruncal regions
• absent thick protrusions from leading edges of cardiomyocytes invading non-myocardial tissue
• cardiomyocytes randomly distributed and mixed with endocardial cells at bases of blood vessels

muscle
• in E13.5 embryos
• cardiomyocytes smaller, rounded and loosely attached to each other with thin cellular projections in conotruncal regions
• absent thick protrusions from leading edges of cardiomyocytes invading non-myocardial tissue
• cardiomyocytes randomly distributed and mixed with endocardial cells at bases of blood vessels




Genotype
MGI:6150918
cn2
Allelic
Composition
Daam1tm1.1Tpy/Daam1tm1.1Tpy
Nkx2-5tm1(cre)Rjs/Nkx2-5+
Genetic
Background
involves: 129S1/Sv * 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Daam1tm1.1Tpy mutation (0 available); any Daam1 mutation (21 available)
Nkx2-5tm1(cre)Rjs mutation (1 available); any Nkx2-5 mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
N
• pulmonary artery and aorta in E16.5 embryos
• in E16.5 embryos
• thicker ventricular trabecular zones, extending into lumen
• inconsistent size of right ventricular trabeculae
• irregular spacing of right ventricular trabeculae
• disorganized left ventricular trabeculae
• left ventricular trabeculae occupy much of ventricular lumen
• in E16.5 embryos
• disorganized
• occupy much of lumen
• thicker trabecular zones
• thinner compact zones
• in E16.5 embryos
• free wall does not extend as close to heart apex of 8-months old mice as in control mice
• base closer to tricuspid valve than in controls
• base not aligned with base of left ventricle
• thicker trabecular zones
• thinner compact zones
• inconsistent size of trabeculae
• irregular spacing of trabeculae
• mean E/A ratio (flow velocity across mitral valve early in diastole divided by that late in diastole) in left ventricle of 2-months old mice is less than 1 in 8-months old mice, compared to greater than 1 in control mice
• in 8-months old mice
• lower acceleration of pulmonary artery (PA) flow, indicating reduced RV contraction force
• reduced fractional shortening
• lower acceleration of pulmonary artery (PA) flow in 8-months old mice

muscle
• in E16.5 embryos
• thicker ventricular trabecular zones, extending into lumen
• inconsistent size of right ventricular trabeculae
• irregular spacing of right ventricular trabeculae
• disorganized left ventricular trabeculae
• left ventricular trabeculae occupy much of ventricular lumen

Mouse Models of Human Disease
DO ID OMIM ID(s) Ref(s)
intrinsic cardiomyopathy DOID:0060036 J:228507




Genotype
MGI:6150919
cn3
Allelic
Composition
Daam1tm1.1Tpy/Daam1tm1.1Tpy
Daam2tm1Tpy/Daam2tm1Tpy
Nkx2-5tm1(cre)Rjs/Nkx2-5+
Genetic
Background
involves: 129S1/Sv * 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Daam1tm1.1Tpy mutation (0 available); any Daam1 mutation (21 available)
Daam2tm1Tpy mutation (0 available); any Daam2 mutation (1 available)
Nkx2-5tm1(cre)Rjs mutation (1 available); any Nkx2-5 mutation (15 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
N
• compact layer width in E17.5 embryos
• in 2-months old mice
• disturbed A-band marker Actc1 striation pattern
• nearly absent intermediate filament Desmin striation
• shorter A-bands
• Z-bands faint or absent
• no apparent I-bands, H-bands or M-lines
• intersecting and disorganized thick fibers in severely affected areas
• lower-density material frequently observed between membranes of adjacent cardiomyocytes in 2-months old mice through electron microscopy
• in E17.5 embryos
• failure of trabeculae to assimilate into compact zone, resulting in occluded ventricular lumens
• wider trabecular layers
• thicker individual trabeculae
• inter-trabecular spaces filled with detached endothelial cells
• in 2-months old mice
• in 2-months old mice
• thicker walls and septa
• smaller lumen
• in 2-months old mice
• thicker walls and septa
• smaller lumen
• in 2-months old mice
• lower ejection fraction
• reduced fractional shortening
• in 2-months old mice
• acceleration of pulmonary artery (PA) flow
• reduced fractional shortening
• increased cardiomyocyte proliferation

muscle
• in 2-months old mice
• disturbed A-band marker Actc1 striation pattern
• nearly absent intermediate filament Desmin striation
• shorter A-bands
• Z-bands faint or absent
• no apparent I-bands, H-bands or M-lines
• intersecting and disorganized thick fibers in severely affected areas
• lower-density material frequently observed between membranes of adjacent cardiomyocytes in 2-months old mice through electron microscopy
• in E17.5 embryos
• failure of trabeculae to assimilate into compact zone, resulting in occluded ventricular lumens
• wider trabecular layers
• thicker individual trabeculae
• inter-trabecular spaces filled with detached endothelial cells
• disturbed A-band marker Actc1 striation pattern and shorter A-bands in cardiomyocytes of 2-months old mice
• no apparent H-bands in cardiomyocytes of 2-months old mice
• no apparent M lines in cardiomyocytes of 2-months old mice
• no apparent I-bands in cardiomyocytes of 2-months old mice
• in cardiomyocytes of some 2-months old mice
• in cardiomyocytes of some 2-months old mice




Genotype
MGI:6150926
cn4
Allelic
Composition
Daam1tm1.1Tpy/Daam1tm1.1Tpy
Nkx2-5tm1(cre)Rjs/Nkx2-5+
Wnt5atm1Amc/Wnt5atm1Amc
Genetic
Background
involves: 129S1/Sv * 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Daam1tm1.1Tpy mutation (0 available); any Daam1 mutation (21 available)
Nkx2-5tm1(cre)Rjs mutation (1 available); any Nkx2-5 mutation (15 available)
Wnt5atm1Amc mutation (1 available); any Wnt5a mutation (6 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• right ventricle shifted anterior to left ventricle in E12.5 embryos
• AV cushion atop single ventricle connected to both atria in some embryos in E12.5 embryos
• rudimentary in left ventricle of E16.5 embryos
• rudimentary in left ventricle of E16.5 embryos
• shorter than controls, not extended to the endocardial cushion in the atrioventricular canal in E12.5 embryos
• mesenchyme-like organization of cardiomyocytes at ventricle base in E12.5 embryos
• right ventricle shifted anterior to left ventricle in E12.5 embryos

mortality/aging
• very few embryos seen at E16.5 and those that are, are mostly necrotic

muscle
• rudimentary in left ventricle of E16.5 embryos
• rudimentary in left ventricle of E16.5 embryos




Genotype
MGI:6151066
cn5
Allelic
Composition
Daam1tm1.1Tpy/Daam1tm1.1Tpy
Nkx2-5tm1(cre)Rjs/Nkx2-5+
Wnt5atm1Amc/Wnt5a+
Genetic
Background
involves: 129S1/Sv * 129S7/SvEvBrd
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Daam1tm1.1Tpy mutation (0 available); any Daam1 mutation (21 available)
Nkx2-5tm1(cre)Rjs mutation (1 available); any Nkx2-5 mutation (15 available)
Wnt5atm1Amc mutation (1 available); any Wnt5a mutation (6 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cardiovascular system
• thicker right ventricular trabecular zones, extending into lumen in E16.5 embryos
• in right ventricle of E16.5 embryos

muscle
• thicker right ventricular trabecular zones, extending into lumen in E16.5 embryos
• in right ventricle of E16.5 embryos





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last database update
03/24/2020
MGI 6.15
The Jackson Laboratory