Phenotypes associated with this allele
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Grid2tm1(cre)Mwa mutation
(0 available);
any
Grid2 mutation
(85 available)
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vision/eye
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• larger spontaneous eye movements
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nervous system
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• mice exhibit normal parallel fiber-interneuron synapses
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behavior/neurological
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• larger spontaneous eye movements
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cacna1atm1Kano mutation
(0 available);
any
Cacna1a mutation
(115 available)
Grid2tm1(cre)Mwa mutation
(0 available);
any
Grid2 mutation
(85 available)
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nervous system
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• regression of surplus climbing fibers (CFs) is severely impaired compared to in control mice
• from P5 to P7, the bias toward strengthening single CFs is impaired compared to in control mice
• multiple CFs translocate to dendrites and impairs elimination of CF synapses from the soma unlike in control cells
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• at P5 to P6, Purkinje cells (PCs) in cerebellar slices exhibit reduced calcium currents compared with control cells
• PCs lack P/Q-type voltage-dependent calcium channel (VDCC) compared with control cells
• climbing fiber (CF)-induced calcium transients in PCs are reduced compared to in control cells
• however, mice exhibit normal P/Q channel contribution to CF to PC synaptic transmission and spontaneous inhibitory postsynaptic currents at P6 to P7 and P10
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• from P5 to P8, the fractions of multiple CF-EPSCs remains unchanged unlike in control mice
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• in climbing fiber cells
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• in climbing fiber cells
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cacna1atm1Kano mutation
(0 available);
any
Cacna1a mutation
(115 available)
Grid2tm1(cre)Mwa mutation
(0 available);
any
Grid2 mutation
(85 available)
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nervous system
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• soma surface of Purkinje cells (PCs) is rough in contrast to smooth surface in controls
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• Purkinje cell (PC) numbers decrease starting at P21 and continue to decrease; soma and dendrites of PCs show degenerative features like somatic shrinkage, darkened cytoplasm, increased electron-dense materials and accumulation of mitochondria with few soma or dendrites found in the cerebellar cortex by P35
• PC degeneration is more apparent in anterior lobules than posterior lobules; remaining PCs display striped or banded patterns
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• surface of Purkinje cell dendrites is rough with protrusion of spines or spine-like processes that form asymmetrical synapses with nerve terminals
• number of spines per 1 um of dendrites (>2 um in caliber) is significantly increased compared to controls
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• terminal profiles in the neuropil are enlarged and in contact with multiple Purkinje cell (PC) spines, often engulfing them, resulting in highly convoluted terminal contours; however density of asymmetrical synapses on PCs is significantly decreased relative to controls
• parallel fiber (PF) terminals are sparse, with many appearing coarse and intense on basis of Slc17a7 (VGluT1) immunolabeling; some PF-PC synapses show a 1:1 contact between PF terminals and PC spines but other exhibit large PF terminals forming multiple contacts
• climbing fiber (CF) terminals are irregular in shape and size; innervation stops midway along proximal dendrites; number of perisomatic CF terminals per unit area of somatic surface is markedly higher than in controls on basis of Slc17a6 (VGluT2) immunolabeling
• regressed innervation territory of climbing fibers on Purkinje cells down to basal dendrites and somata is observed in mutants resulting in multiple innervation
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• cerebellum is smaller than in controls at P21, but foliation and laminar organization as well as dendritic branching appear normal
• cerebellum size is reduced even more from P21 to adult
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• unitary EPSCs fromPurkinje cells have larger and more variable amplitudes than controls
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• paired-pulse ratio at short interpulse intervals is smaller than in controls indicating a higher presynaptic release probability
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