All Phenotypes Drosha<tm1.1Litt> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Drosha^tm1Litt/Drosha^tm1.1Litt Gt(ROSA)26Sor^{tm1(cre/ERT)Nat}/Gt(ROSA)26Sor⁺	involves: 129 * 129P2/OlaHsd * C57BL/6 * FVB/N	MGI:3806251
cn2	Drosha^tm1Litt/Drosha^tm1.1Litt Tg(Cd4-cre)1Cwi/0	involves: 129P2/OlaHsd * C57BL/6 * DBA/2 * FVB/N	MGI:3806244
cn3	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Y	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	MGI:3806253
cn4	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr}	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N	MGI:3806254

Allelic Composition	Drosha^tm1Litt/Drosha^tm1.1Litt Gt(ROSA)26Sor^{tm1(cre/ERT)Nat}/Gt(ROSA)26Sor⁺
Genetic Background	involves: 129 * 129P2/OlaHsd * C57BL/6 * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Drosha^tm1.1Litt mutation (0 available); any Drosha mutation (96 available)
Drosha^tm1Litt mutation (1 available); any Drosha mutation (96 available)
Gt(ROSA)26Sor^{tm1(cre/ERT)Nat} mutation (3 available); any Gt(ROSA)26Sor mutation (944 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal interferon-gamma secretion ( J:138683 )

• naive CD4+ T cells release more interferon gamma than controls after cre-mediated deletion of Rnasen induced by tamoxifen treatment

• deficient CD4+ T cells are capable of differentiating into Th1 or Th2 cells

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:138683 )

• cachexic animals become moribund rapidly and are euthanized; by 6 months, 50% mortality in mutants is observed

growth/size/body

decreased body fat mass ( J:138683 )

• moribund animals show complete loss of visceral adipose tissue

cachexia ( J:138683 )

• displayed by many animals at 4 months

immune system

immune system phenotype ( J:138683 )

N	• spleen and lymph nodes are not larger than controls despite T cell abnormalities

increased granulocyte number ( J:138683 )

• higher frequency of granulocytes in spleen and lymph nodes is observed

increased gamma-delta T cell number ( J:138683 )

• increase is detected in spleen and lymph nodes

increased activated T cell number ( J:138683 )

• significant increase in frequency of activated (CD62L^lo44^hi) CD4+ T cells is observed in spleen and lymph nodes

decreased lymphocyte cell number ( J:138683 )

• T lymphopenia in CD8+ compartment is observed

decreased thymocyte number ( J:138683 )

• total thymocyte number is decreased compared to controls at 6 weeks; reduced frequency of TCRbeta+ thymocytes with downregulated CD14 and CD69 is observed

• absolute number of mature thymocytes is significantly reduced

decreased double-positive T cell number ( J:138683 )

• absolute number of double positive (DP) thymocytes is significantly reduced compared to controls at 6 weeks

decreased CD8-positive, alpha-beta T cell number ( J:138683 )

• significant reduction in frequency of mature CD8+ T cells is observed in periphery at 6 weeks

decreased regulatory T cell number ( J:138683 )

• mature peripheral T reg cells are reduced in frequency

abnormal immune system physiology ( J:138683 )

• very high frequencies of interferon gamma- or IL-17A-secreting cells are observed; high frequency of interferon gamma/IL-17A double secreting cells is detected in moribund animals

• slight increase in frequency of interferon gamma- or IL-17A-secreting CD4+ T cells can be seen at 3 months

• regulatory T cells have reduced suppressive activity compared to controls; in vitro proliferation of stimulated CD25-CD4+ T cells is only partially suppressed

• deficient CD4+ T cells are capable of differentiating into Th1 or Th2 cells

intestinal inflammation ( J:138683 )

• small foci of inflammatory cells are more prevalent than in controls

liver inflammation ( J:138683 )

• infiltrating cells accumulate around most blood vessels in liver with additional foci in sinusoids

lung inflammation ( J:138683 )

• infiltrating cells accumulate primarily around blood vessels, resulting in epithelial thickening of surrounding tissue

hematopoietic system

increased granulocyte number ( J:138683 )

• higher frequency of granulocytes in spleen and lymph nodes is observed

increased gamma-delta T cell number ( J:138683 )

• increase is detected in spleen and lymph nodes

increased activated T cell number ( J:138683 )

• significant increase in frequency of activated (CD62L^lo44^hi) CD4+ T cells is observed in spleen and lymph nodes

decreased lymphocyte cell number ( J:138683 )

• T lymphopenia in CD8+ compartment is observed

decreased thymocyte number ( J:138683 )

• total thymocyte number is decreased compared to controls at 6 weeks; reduced frequency of TCRbeta+ thymocytes with downregulated CD14 and CD69 is observed

• absolute number of mature thymocytes is significantly reduced

decreased double-positive T cell number ( J:138683 )

• absolute number of double positive (DP) thymocytes is significantly reduced compared to controls at 6 weeks

decreased CD8-positive, alpha-beta T cell number ( J:138683 )

• significant reduction in frequency of mature CD8+ T cells is observed in periphery at 6 weeks

decreased regulatory T cell number ( J:138683 )

• mature peripheral T reg cells are reduced in frequency

adipose tissue

decreased body fat mass ( J:138683 )

• moribund animals show complete loss of visceral adipose tissue

liver/biliary system

liver inflammation ( J:138683 )

• infiltrating cells accumulate around most blood vessels in liver with additional foci in sinusoids

respiratory system

lung inflammation ( J:138683 )

• infiltrating cells accumulate primarily around blood vessels, resulting in epithelial thickening of surrounding tissue

muscle

decreased skeletal muscle mass ( J:138683 )

• moribund animals exhibit reduction in muscle mass

digestive/alimentary system

intestinal inflammation ( J:138683 )

• small foci of inflammatory cells are more prevalent than in controls

endocrine/exocrine glands

decreased thymocyte number ( J:138683 )

• total thymocyte number is decreased compared to controls at 6 weeks; reduced frequency of TCRbeta+ thymocytes with downregulated CD14 and CD69 is observed

• absolute number of mature thymocytes is significantly reduced

Allelic Composition	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Y
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Drosha^tm1.1Litt mutation (0 available); any Drosha mutation (96 available)
Drosha^tm1Litt mutation (1 available); any Drosha mutation (96 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:138683 )

• males become ill at 2-3 weeks, exhibiting 100% mortality at <25 days

cardiovascular system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

hematopoietic system

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

immune system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

enlarged lymph nodes ( J:138683 )

• moribund mice display massive lymphadenopathy at 2.5-3 weeks of age

abnormal interferon-gamma secretion ( J:138683 )

• hyper-Ifng secretion by CD4+ and CD8+ T cells is observed

abnormal interleukin-4 secretion ( J:138683 )

• hyper-IL-4 secretion by CD4+ T cells is observed

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

liver/biliary system

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

respiratory system

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

growth/size/body

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

Allelic Composition	Drosha^tm1Litt/Drosha^tm1.1Litt Foxp3^{tm4(YFP/icre)Ayr}/Foxp3^{tm4(YFP/icre)Ayr}
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * FVB/N

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Drosha^tm1.1Litt mutation (0 available); any Drosha mutation (96 available)
Drosha^tm1Litt mutation (1 available); any Drosha mutation (96 available)
Foxp3^{tm4(YFP/icre)Ayr} mutation (2 available); any Foxp3 mutation (55 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:138683 )

• females become ill at 2-3 weeks, exhibiting 100% mortality at <25 days

immune system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal regulatory T cell morphology ( J:138683 )

• decrease in Foxp3+ CD25^lo population

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

enlarged lymph nodes ( J:138683 )

• moribund mice display massive lymphadenopathy at 2.5-3 weeks of age

abnormal interferon-gamma secretion ( J:138683 )

• hyper-Ifng secretion by CD4+ and CD8+ T cells is observed

abnormal interleukin-4 secretion ( J:138683 )

• hyper-Il-4 secretion by CD4+ T cells is observed

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

hematopoietic system

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

increased leukocyte cell number ( J:138683 )

• all leukocyte populations are expanded equally, except for consistent increase observed in CD8+ T cell number

increased CD8-positive, alpha-beta T cell number ( J:138683 )

abnormal regulatory T cell morphology ( J:138683 )

• decrease in Foxp3+ CD25^lo population

abnormal T cell activation ( J:138683 )

• increased frequencies of CD62L^lo44^hi CD4+ and CD8+ T cells are observed, indicating aberrant activation

cardiovascular system

blood vessel inflammation ( J:138683 )

• cuffing around blood vessels by inflammatory cells in pancreas, kidney and skeletal muscle is observed

liver/biliary system

liver inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

respiratory system

lung inflammation ( J:138683 )

• large areas are infiltrated by inflammatory cells in moribund mice

growth/size/body

enlarged spleen ( J:138683 )

• moribund mice display splenomegaly

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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