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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Gad1tm1Rpa
targeted mutation 1, Richard D Palmiter
MGI:3527168
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Gad1tm1Rpa/Gad1tm1Rpa Not Specified MGI:3762151
cn2
Gad1tm1Rpa/Gad1tm1Rpa Not Specified MGI:3762153
cn3
Gad1tm1Rpa/Gad1+ Not Specified MGI:3762154
cn4
Drd1tm1(cre)Rpa/Drd1+
Gad1tm1Rpa/Gad1tm1.1Rpa
involves: 129S4/SvJaeSor * C57BL/6 MGI:3806595
cn5
Gad1tm1.1Bgc/Gad1tm1Rpa
Tg(Nes-cre)1Kln/0
involves: 129S6/SvEvTac * C57BL/6 * SJL MGI:4442419


Genotype
MGI:3762151
hm1
Allelic
Composition
Gad1tm1Rpa/Gad1tm1Rpa
Genetic
Background
Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gad1tm1Rpa mutation (1 available); any Gad1 mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
normal phenotype
• mice are phenotypically normal




Genotype
MGI:3762153
cn2
Allelic
Composition
Gad1tm1Rpa/Gad1tm1Rpa
Genetic
Background
Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gad1tm1Rpa mutation (1 available); any Gad1 mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• following transfection with a cre-recombinase vector, the axons of basket interneurons are thinner and have reduced numbers of finer branches especially terminal branches compared to in control cells
• following transfection with a cre-recombinase vector, terminal branches have fewer and smaller boutons around pyramidal cell somata throughout the innervation field (2.7+/-0.4 boutons per soma compared to 8.9+/-0.7 boutons per soma in control cells)
• following transfection with a cre-recombinase vector, it is difficult to identify synaptic vesicles and synaptic specialization unlike in control interneurons
• following transfection with a cre-recombinase vector, basket interneurons display reduced axon density and percentage of targeted neuronal somatafollowing transfection with a cre-recombinase vector, basket interneurons display reduced axon density and percentage of targeted neuronal somata
• however, following transfection with a cre-recombinase vector, the shape and gross branching patterns are normal
• however, baclofen treatment following transfection with a cre-recombinase vector partially resuces bouton density (6.1+/-0.5 boutons per soma compared to 2.7+/-0.4 in untreated cells)
• following infection with an adenovirus-cre, basket interneurons in the visual cortex have thinner axon terminals, frequent stretches without boutons around pyramidal cell somata, and smaller perisomatic bouton size (40% reduction in bouton diameter)




Genotype
MGI:3762154
cn3
Allelic
Composition
Gad1tm1Rpa/Gad1+
Genetic
Background
Not Specified
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gad1tm1Rpa mutation (1 available); any Gad1 mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
nervous system
• following infection with an andenovirus-cre, the axons of basket interneurons are thinner and have reduced numbers of finer branches especially terminal branches compared to in control cells
• following infection with an andenovirus-cre, terminal branches have fewer and smaller boutons around pyramidal cell somata throughout the innervation field (5.0+/-0.3 boutons per soma compared to 8.9+/-0.4 boutons per soma in control cells)
• however, NO711 treatment following infection with an andenovirus-cre rescues perisomatic bouton formation and increases complexity of ternimal branches, bouton density, and size of perisomatic boutons while treatment with diazepam treated cells show much less pronounced perisomatic synapse defects




Genotype
MGI:3806595
cn4
Allelic
Composition
Drd1tm1(cre)Rpa/Drd1+
Gad1tm1Rpa/Gad1tm1.1Rpa
Genetic
Background
involves: 129S4/SvJaeSor * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Drd1tm1(cre)Rpa mutation (0 available); any Drd1 mutation (27 available)
Gad1tm1.1Rpa mutation (0 available); any Gad1 mutation (18 available)
Gad1tm1Rpa mutation (1 available); any Gad1 mutation (18 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
behavior/neurological
N
• mice exhibit normal gait and response to the Drd1a agonist SKF81297
• mice spend more time on a rotarod than heterozygous controls
• on the second of three trials mice exhibit decreased strength in a modified wire hang test compared to heterozygous controls




Genotype
MGI:4442419
cn5
Allelic
Composition
Gad1tm1.1Bgc/Gad1tm1Rpa
Tg(Nes-cre)1Kln/0
Genetic
Background
involves: 129S6/SvEvTac * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gad1tm1.1Bgc mutation (1 available); any Gad1 mutation (18 available)
Gad1tm1Rpa mutation (1 available); any Gad1 mutation (18 available)
Tg(Nes-cre)1Kln mutation (4 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
craniofacial
• at E17.5 in 9 of 11 mice

embryo
• umbilical hernia is seen at E17.5 in 6 of 11 mice

digestive/alimentary system
• at E17.5 in 9 of 11 mice

growth/size/body
• at E17.5 in 9 of 11 mice





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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
10/08/2019
MGI 6.14
The Jackson Laboratory