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Allele Symbol Allele Name Allele ID |
Tg(Cyp1a1-cre)1Dwi transgene insertion 1, Douglas J Winton MGI:3052655 |
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| Summary |
12 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• increased proliferation of columnar epithelial cells of the villi
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• increased proliferation of columnar epithelial cells of the villi
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 3 and 5 days after injection with beta-naphthoflavone the number of goblet cells is increased in the small intestine and colon
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• 5 days after injection with beta-naphthoflavone, essentially all epithelial cell division has stopped in the small intestine and colon, but no significant increase in apoptosis is seen
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• 5 days after injection with beta-naphthoflavone, in the small intestine and colon the rapidly dividing transit amplifying compartment is completely replaced by post-mitotic goblet cells
• however, in the small intestine Paneth cell morphology is normal
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• 5 days after injection with beta-naphthoflavone, in the small intestine and colon the rapidly dividing transit amplifying compartment is completely replaced by post-mitotic goblet cells
• however, in the small intestine Paneth cell morphology is normal
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• 3 and 5 days after injection with beta-naphthoflavone the number of goblet cells is increased in the small intestine and colon
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• cre induction leads to an increase in crypt cell apoptosis
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• five days after cre-induction, the elongated Olfm4+ stem cells at the base of crypts are absent
• by 15 days after cre-induction, stem cells that have escaped recombination of the floxed allele have completely repopulated the crypts
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• five days after cre-induction, the elongated Olfm4+ stem cells at the base of crypts are absent
• by 15 days after cre-induction, stem cells that have escaped recombination of the floxed allele have completely repopulated the crypts
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• cre induction leads to an increase in crypt cell apoptosis
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in mice induced with beta-naphthoflavonein mice induced with beta-naphthoflavonein mice induced with beta-naphthoflavone
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• in mice induced with beta-naphthoflavone
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• in one mouse induced with beta-naphthoflavone
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• in one mouse induced with beta-naphthoflavone
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• in mice induced with beta-naphthoflavone
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• mice induced with beta-naphthoflavone exhibit atypical endometrial hyperplasia
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• in mice induced with beta-naphthoflavone leading to euthanasia
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| N |
• mice induced with beta-naphthoflavone exhibit normal crypt-villus architecture and physiology
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• in mice induced with beta-naphthoflavonein mice induced with beta-naphthoflavonein mice induced with beta-naphthoflavone
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice exhibit normal circulating liver enzyme, liver size, and liver cell proliferation
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in beta-napthoflavone-treated mice
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• after 5 days, beta-napthoflavone-treated mice exhibit a loss of crypt width with loss of crypt stem cells and proliferating progenitors compared with control mice
• however, wild-type crypt stem cell escapers exhibit proliferation
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• in beta-napthoflavone-treated mice
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• after 5 days, beta-napthoflavone-treated mice exhibit a loss of crypt width with loss of crypt stem cells and proliferating progenitors compared with control mice
• however, wild-type crypt stem cell escapers exhibit proliferation
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• in beta-napthoflavone-treated mice
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• beta-napthoflavone-treated mice exhibit a loss of crypt width with loss of crypt stem cells and proliferating progenitors compared with control mice
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• in beta-napthoflavone-treated mice
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• beta-napthoflavone-treated mice exhibit a loss of crypt width with loss of crypt stem cells and proliferating progenitors compared with control mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• beta-napthoflavone-treated mice exhibit normal crypt width and crypt stem cell proliferation
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• 4 days after BNF treatment increased apoptosis is seen in crypt epithelium
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• 4 days after BNF treatment the number of crypts is reduced however by 5 days post treatment surviving crypts with unrecombined cells are enlarged and show increased proliferation
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• the number of Paneth cells is reduced within the crypt 4 days after BNF treatment
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• Paneth cells are in abnormal locations within the crypt 4 days after BNF treatment
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• 4 days after BNF treatment the number of crypts is reduced however by 5 days post treatment surviving crypts with unrecombined cells are enlarged and show increased proliferation
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• the number of Paneth cells is reduced within the crypt 4 days after BNF treatment
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• Paneth cells are in abnormal locations within the crypt 4 days after BNF treatment
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• at P8
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• reduce in polysome loading at P4 in the liver due to shortage of tRNA
• absent polysome at P8
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• reduce in polysome loading at P4 in the liver due to shortage of tRNA
• absent polysome at P8
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mice develop similar tumor number and size as in Apctm1aTno Tg(Cyp1a1-cre)1Dwi heterozygotes
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• mice develop similar tumor number and size as in Apctm1aTno Tg(Cyp1a1-cre)1Dwi heterozygotes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• the number of cleaved CASPASE-3-positive small intestine crypt cells is increased at 0, 2, 4, and 7 days after beta-napthoflavone (betaNF) treatment
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• the number of BrdU-positive small intestine crypt cells is reduced at day 2 but increased by day 4 post-betaNF treatment
• similarly, the number of BrdU/Krt8-double positive crypt cells per small intestine length is reduced at day 2 but increased by day 4 post-betaNF treatment
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• at day 2 after the last of 5 consecutive beta-napthoflavone (betaNF) injections, small intestine architecture is severely disrupted, with fewer and smaller crypts and shorter, hypocellular and progressively disorganized villi
• however, by day 4 after the last betaNF injection, ORC1 protein levels are restored to nearly normal levels and a 4- to 5-day repopulation period ensues with enhanced DNA replication, progenitor cell proliferation and crypt hypertrophy such that crypt-villus integrity is fully restored at day 7 post-treatment
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• at day 2 post-betaNF treatment, fewer and smaller crypts are observed in the small intestine
• however, crypt integrity is fully restored at day 7 post-betaNF treatment
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• at day 2 post-betaNF treatment, small intestine villi are shorter, hypocellular and progressively disorganized
• however, villus integrity is fully restored at day 7 post-betaNF treatment
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• small intestine villi are shorter at day 2 but not at day 7 post-betaNF treatment
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• at day 2 post-betaNF treatment, fewer and smaller crypts are observed in the small intestine
• however, crypt integrity is fully restored at day 7 post-betaNF treatment
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• the number of cleaved CASPASE-3-positive small intestine crypt cells is increased at 0, 2, 4, and 7 days after beta-napthoflavone (betaNF) treatment
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• the number of BrdU-positive small intestine crypt cells is reduced at day 2 but increased by day 4 post-betaNF treatment
• similarly, the number of BrdU/Krt8-double positive crypt cells per small intestine length is reduced at day 2 but increased by day 4 post-betaNF treatment
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• DNA replication is enhanced during the 4- to 5-day repopulation period that follows after acute disruption of intestinal integrity
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• DNA replication is enhanced during the 4- to 5-day repopulation period that follows after acute disruption of intestinal integrity
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 04/16/2024 MGI 6.23 |
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