Phenotypes associated with this allele

• Allele Symbol: Tg(ACTB-NOTCH1)1Shn
• Allele Name: transgene insertion 1, Jie Shen
• Allele ID: MGI:3044583
• Summary: 5 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Tg(Nes-cre)1Kln/0 Tg(ACTB-NOTCH1)1Shn/0	B6.Cg-Tg(Nes-cre)1Kln Tg(ACTB-NOTCH1)1Shn	MGI:3044595
cn2	Tg(ACTB-NOTCH1)1Shn/0 Tg(Nes-cre)1Kln/0 Trp53^tm1Tyj/Trp53^tm1Tyj	involves: 129S2/SvPas * C57BL/6J	MGI:3044601
cn3	Tg(ACTB-NOTCH1)1Shn/0 Tg(Nes-cre)1Kln/0 Trp53^tm1Tyj/Trp53^+	involves: 129S2/SvPas * C57BL/6J	MGI:3044602
cn4	Tg(ACTB-NOTCH1)1Shn/? Tg(Nes-cre/Esr1*)4Ynj/?	involves: C57BL/6 * C57BL/6J	MGI:3765969
cn5	Tg(ACTB-NOTCH1)1Shn/0 Tg(Syn1-cre)671Jxm/0	involves: C57BL/6J	MGI:3044599

Genotype
MGI:3044595

cn1

• Allelic Composition: Tg(Nes-cre)1Kln/0; Tg(ACTB-NOTCH1)1Shn/0
• Genetic Background: B6.Cg-Tg(Nes-cre)1Kln Tg(ACTB-NOTCH1)1Shn

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

perinatal lethality, complete penetrance ( J:90392 )

prenatal lethality, incomplete penetrance ( J:90392 )

• some of the mutants die before birth; however, most survive to the perinatal stage

nervous system

abnormal neuron apoptosis ( J:90392 )

• at E10 the number of apoptotic cells in the forebrain-midbrain junction is increased

• by E11.5 apoptosis is more widespread in the telencephalon with about 30% of all cells being TUNEL positive

abnormal cortical ventricular zone morphology ( J:90392 )

• at E12 - 13.5 the ventricular zone harboring neural progenitors is thinner

small embryonic telencephalon ( J:90392 )

• at E12 - 13.5 the telencephalon is smaller compared to wild-type or single transgenic littermates

decreased brain size ( J:90392 )

• at E12 - 13.5 all brain subregions are smaller compared to wild-type or single transgenic littermates

• at P0 the brain especially the cerebral cortex is much smaller in double transgenic mice compared to wild-type or single transgenic littermates

decreased neuron number ( J:90392 )

• the number of postmitotic neurons is decreased at E12 - 13.5 in the telencephalon and diencephalon

cellular

abnormal neuron apoptosis ( J:90392 )

• at E10 the number of apoptotic cells in the forebrain-midbrain junction is increased

• by E11.5 apoptosis is more widespread in the telencephalon with about 30% of all cells being TUNEL positive

Genotype
MGI:3044601

cn2

• Allelic Composition: Tg(ACTB-NOTCH1)1Shn/0; Tg(Nes-cre)1Kln/0; Trp53^tm1Tyj/Trp53^tm1Tyj
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

mortality/aging

prenatal lethality, incomplete penetrance ( J:90392 )

• fewer than expected double transgenic homozygous mutants are found due to incomplete penetrance of Trp53^tm1Tyj embryonic lethality

cellular

cellular phenotype ( J:90392 )

N • at E10 - 11.5 no increase in the number of apoptotic cells in the brain is found unlike in double transgenics with wild-type Trp53

Genotype
MGI:3044602

cn3

• Allelic Composition: Tg(ACTB-NOTCH1)1Shn/0; Tg(Nes-cre)1Kln/0; Trp53^tm1Tyj/Trp53⁺
• Genetic Background: involves: 129S2/SvPas * C57BL/6J

cellular

abnormal apoptosis ( J:90392 )

• at E10 - 11.5 the number of apoptotic cells in the brain is increased

Genotype
MGI:3765969

cn4

• Allelic Composition: Tg(ACTB-NOTCH1)1Shn/?; Tg(Nes-cre/Esr1*)4Ynj/?
• Genetic Background: involves: C57BL/6 * C57BL/6J

nervous system

nervous system phenotype ( J:127227 )

N • no enlargement of lateral ventricles or defects in ependymal adhesion of ventricular walls are observed

increased neuron apoptosis ( J:127227 )

• overexpression of Notch1 in induced mutants results in significantly increased Doublecortin-positive neuroblast apoptosis at the striato-cortical junction

abnormal postnatal subventricular zone morphology ( J:127227 )

• overexpression of Notch1 in induced mutants results in significantly increased Doublecortin-positive neuroblast cellularity at the striato-cortical junction

cellular

increased neuron apoptosis ( J:127227 )

• overexpression of Notch1 in induced mutants results in significantly increased Doublecortin-positive neuroblast apoptosis at the striato-cortical junction

Genotype
MGI:3044599

cn5

• Allelic Composition: Tg(ACTB-NOTCH1)1Shn/0; Tg(Syn1-cre)671Jxm/0
• Genetic Background: involves: C57BL/6J

cellular

cellular phenotype ( J:90392 )

N • no increase in apoptosis is seen at E13.5 in the telencephalon of double transgenic mice