Analysis Tools
nervous system
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• facilitation of cortical synaptic transmission induced by 200 micromolar acetylcholine, measured the changes in amplitude of extracellular field potentials (FPs) evoked by stimulation of white matter (WM) and recording in
cortical layer II/III, is similar to controls
• depression of cortical synaptic transmission induced by 1.5mm acetylcholine is not affected
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• muscarine-induced gamma oscillations are absent in the hippocampus
• individual pyramidal neurons show only a modest muscarine-induced depolarization, which is delayed in onset compared to wild-type
• muscarinic modulation of the calcium-dependent nonspecific cation conductance, but not the voltage- and time-dependent potassium current, is lost in mutant pyramidal neurons
• modulation of the hyperpolarization-activated mixed cation current by muscarine is absent in pyramidal neurons
• muscarine does not increase the hyperpolarization-activated mixed cation current conductance in mutant pyramidal neurons as in wild-type
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respiratory system
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• muscarine causes significantly stronger bronchoconstriction responses in mutant bronchi at low concentrations (10-8 M and 10-6M) but the response is similar to controls at high concentration (10-4M)
• the transient bronchorelaxation response after the initial bronchoconstriction that is seen in control preparations is absent in smaller bronchi from mutant mice
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immune system
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• CD8+ T cells derived from mutant mice develop virtually no detectable lytic activity upon antibody stimulation; however in a time course study, CD8+ T cells from the mutant animals acquired lytic activity after 3 days of stimulation, but this activity was less than control cells, suggesting that mutant mice have both delayed kinetics and an increased
activation threshold for CTL development
• the reduced lytic activity in the mutant mice was not due to a quantitative defect in CD8+ T cell development, since splenocytes and thymocytes from mutant and control mice had equivalent numbers of CD8+ T cells
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homeostasis/metabolism
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• muscarine causes significantly stronger bronchoconstriction responses in mutant bronchi at low concentrations (10-8 M and 10-6M) but the response is similar to controls at high concentration (10-4M)
• the transient bronchorelaxation response after the initial bronchoconstriction that is seen in control preparations is absent in smaller bronchi from mutant mice
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hematopoietic system
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• CD8+ T cells derived from mutant mice develop virtually no detectable lytic activity upon antibody stimulation; however in a time course study, CD8+ T cells from the mutant animals acquired lytic activity after 3 days of stimulation, but this activity was less than control cells, suggesting that mutant mice have both delayed kinetics and an increased
activation threshold for CTL development
• the reduced lytic activity in the mutant mice was not due to a quantitative defect in CD8+ T cell development, since splenocytes and thymocytes from mutant and control mice had equivalent numbers of CD8+ T cells
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