Phenotypes associated with this allele

• Allele Symbol: Mtf1^tm1Wsc
• Allele Name: targeted mutation 1, Walter Schaffner
• Allele ID: MGI:2182939
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Mtf1^tm1Wsc/Mtf1^tm1Wsc	involves: 129 * C57BL/6	MGI:2656942
hm2	Mtf1^tm1Wsc/Mtf1^tm1Wsc	Not Specified	MGI:3046549
cn3	Mtf1^tm1Wsc/Mtf1^tm1Wsc Tg(Mx1-cre)1Cgn/0 Tg(UBC-Mtf1)1Wsc/0	involves: 129/Sv * C57BL/6 * CBA	MGI:3046551
cx4	Mtf1^tm1Wsc/Mtf1^tm1Wsc Tg(UBC-Mtf1)1Wsc/0	Not Specified	MGI:3046550

Genotype
MGI:2656942

hm1

• Allelic Composition: Mtf1^tm1Wsc/Mtf1^tm1Wsc
• Genetic Background: involves: 129 * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:48216 )

• normal ratio of homozygotes present up to E13.5 but no viable embryos after E14.5

liver/biliary system

hepatic necrosis ( J:48216 )

• although liver size was normal and there were no indications of apoptosis, liver damage was present at E13.5

• severe liver necrosis at E14.5 with diffuse bleeding and generalized edema in the only surviving embryo examined at E14.5

cellular

increased cellular sensitivity to alkylating agents ( J:48216 )

• increased susceptibility of embryo fibroblasts to cadmium and hydrogen peroxide

hepatic necrosis ( J:48216 )

• although liver size was normal and there were no indications of apoptosis, liver damage was present at E13.5

• severe liver necrosis at E14.5 with diffuse bleeding and generalized edema in the only surviving embryo examined at E14.5

Genotype
MGI:3046549

hm2

• Allelic Composition: Mtf1^tm1Wsc/Mtf1^tm1Wsc
• Genetic Background: Not Specified

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:91119 )

• embryos die around E14 due to liver degeneration

liver/biliary system

dilated liver sinusoidal space ( J:91119 )

hepatic necrosis ( J:91119 )

• embryonic liver degeneration with irregular patches of necrotic cells is seen

• mutant hepatocytes in primary culture are less viable compared to wild-type hepatocytes

cardiovascular system

dilated liver sinusoidal space ( J:91119 )

cellular

hepatic necrosis ( J:91119 )

• embryonic liver degeneration with irregular patches of necrotic cells is seen

• mutant hepatocytes in primary culture are less viable compared to wild-type hepatocytes

Genotype
MGI:3046551

cn3

• Allelic Composition: Mtf1^tm1Wsc/Mtf1^tm1Wsc; Tg(Mx1-cre)1Cgn/0; Tg(UBC-Mtf1)1Wsc/0
• Genetic Background: involves: 129/Sv * C57BL/6 * CBA

mortality/aging

increased susceptibility to xenobiotic induced morbidity/mortality ( J:91119 )

• exposure to cadmium was lethal for all male and 2 out of 3 female mutants after induction of cre mediated recombination compared to no male and only 1 female (out of 4) death in mutants without the cre transgene

homeostasis/metabolism

increased susceptibility to xenobiotic induced morbidity/mortality ( J:91119 )

• exposure to cadmium was lethal for all male and 2 out of 3 female mutants after induction of cre mediated recombination compared to no male and only 1 female (out of 4) death in mutants without the cre transgene

increased physiological sensitivity to xenobiotic ( J:91119 )

• cadmium exposure resulted in cell degeneration and necrosis in the liver, lung, and pancreas in all male mutants and some of the female mutants after induction of cre mediated recombination

abnormal xenobiotic pharmacokinetics ( J:91119 )

• transgenic mice after induction of cre accumulate less cadmium in the liver and kidneys compared to control transgenic mice that do not carry the cre transgene

immune system

decreased leukocyte cell number ( J:91119 )

• transgenic mice after induction of cre have one third of the number of white blood cells compared to control transgenic mice that do not carry the cre transgene

decreased lymphocyte cell number ( J:91119 )

• the lymphocyte population in particular is severely reduced in transgenic mice after induction of cre compared to control transgenic mice that do not carry the cre transgene

hematopoietic system

decreased leukocyte cell number ( J:91119 )

• transgenic mice after induction of cre have one third of the number of white blood cells compared to control transgenic mice that do not carry the cre transgene

decreased lymphocyte cell number ( J:91119 )

• the lymphocyte population in particular is severely reduced in transgenic mice after induction of cre compared to control transgenic mice that do not carry the cre transgene

Genotype
MGI:3046550

cx4

• Allelic Composition: Mtf1^tm1Wsc/Mtf1^tm1Wsc; Tg(UBC-Mtf1)1Wsc/0
• Genetic Background: Not Specified

normal phenotype

no abnormal phenotype detected ( J:91119 )

• presence of the transgene rescues the null phenotype