Mtf1^tm1Wsc
Targeted Allele Detail

Summary

• Symbol: Mtf1^tm1Wsc
• Name: metal response element binding transcription factor 1; targeted mutation 1, Walter Schaffner
• MGI ID: MGI:2182939
• Synonyms: MTF-1^-
• Gene: Mtf1 Location: Chr4:124696342-124743593 bp, + strand Genetic Position: Chr4, 57.91 cM, cytoband D1
• Alliance: Mtf1^tm1Wsc page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:48216
• Parent Cell Line: GS1 (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A neomycin selection cassette replaced sequences encoding the first two and a portion of the third zinc-finger domain. RT-PCR analysis of RNA from mutant embryos revealed the presence of an amino-terminal fragment, truncating 39 base pairs downstream of the 5' insertion point. The truncated transcript lacks sequence encoding 3 zinc-finger domains and the acidic, proline-rich, and serine/threonine-rich regions. (J:19100, J:48216)

Expression

In Mice Carrying this Mutation:	28 assay results
In Structures Affected by this Mutation:	1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Mtf1 Mutation: 39 strains or lines available

References

• Original: J:48216 Gunes C, et al., Embryonic lethality and liver degeneration in mice lacking the metal-responsive transcriptional activator MTF-1. EMBO J. 1998 May 15;17(10):2846-54
• All: 6 reference(s)