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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Slit2tm1Matl
targeted mutation 1, Marc Tessier-Lavigne
MGI:2179460
Summary 12 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Slit2tm1Matl/Slit2tm1Matl involves: 129S2/SvPas MGI:3043167
cn2
 		Ext1tm1Yama/Ext1+
Slit2tm1Matl/Slit2tm1Matl
Tg(Nes-cre)1Kln/0 		involves: 129S2/SvPas * 129S5/SvEvBrd * C57BL/6 * SJL MGI:2682063
cx3
 		Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg
Slit2tm1Matl/Slit2tm1Matl 		involves: 129P2/OlaHsd * 129S2/SvPas MGI:5522645
cx4
 		Slit1tm1.1Matl/Slit1tm1.1Matl
Slit2tm1Matl/Slit2tm1Matl
Slit3tm1.1Dor/Slit3tm1.1Dor 		involves: 129S1/Sv * 129S2/SvPas MGI:3043188
cx5
 		Slit2tm1Matl/Slit2tm1Matl
Slit3tm1.1Dor/Slit3tm1.1Dor 		involves: 129S1/Sv * 129S2/SvPas MGI:5522767
cx6
 		Slit1tm1.1Matl/Slit1tm1.1Matl
Slit2tm1Matl/Slit2tm1Matl
Slit3tm1.1Dor/Slit3+ 		involves: 129S1/Sv * 129S2/SvPas MGI:3043191
cx7
 		Gdnftm1Rosl/Gdnf+
Slit2tm1Matl/Slit2tm1Matl 		involves: 129S2/SvPas MGI:3043170
cx8
 		Slit1tm1.1Matl/Slit1tm1.1Matl
Slit2tm1Matl/Slit2+ 		involves: 129S2/SvPas MGI:5522757
cx9
 		Slit1tm1.1Matl/Slit1+
Slit2tm1Matl/Slit2tm1Matl 		involves: 129S2/SvPas MGI:5522762
cx10
 		Slit1tm1.1Matl/Slit1tm1.1Matl
Slit2tm1Matl/Slit2tm1Matl 		involves: 129S2/SvPas MGI:3772917
cx11
 		Robo3tm1Matl/Robo3tm1Matl
Slit1tm1.1Matl/Slit1tm1.1Matl
Slit2tm1Matl/Slit2tm1Matl 		involves: 129/Sv * CD-1 MGI:3043203
cx12
 		Robo3tm1Matl/Robo3tm1Matl
Slit2tm1Matl/Slit2tm1Matl 		involves: 129/Sv * CD-1 MGI:3043199


Genotype
MGI:3043167
hm1
Allelic
Composition		 Slit2tm1Matl/Slit2tm1Matl
Genetic
Background		 involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slit2tm1Matl mutation (2 available); any Slit2 mutation (88 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
perinatal lethality, incomplete penetrance ( J:90324 )
• almost all homozygotes die at birth

cellular
abnormal cell adhesion ( J:106011 )
• cell adhesion defects are seen in end buds and ducts of transplanted mammary glands

endocrine/exocrine glands
abnormal mammary gland duct morphology ( J:106011 )
• end buds formed by transplanted mammary analage display enlarged subcapsular spaces, invaginated cap cell layers and disorganized luminal epithelial cells
• sporadic loss of luminal epithelial cells and separation of epithelial cell layers is detected in transplanted mammary glands

renal/urinary system
abnormal kidney development ( J:90324 )
• many of the multiple ureteric buds have developed to give rise to 2 or more adjacent kidneys
• fusion of these adjacent kidneys may cause the interior nephrogenesis seen at E18.5
abnormal nephrogenic zone morphology ( J:90324 )
• at E18.5 the region where new nephrons are being generated is not restricted to the periphery as in wild-type mice but extends into the interior of the kidney in homozygotes
abnormal ureter morphology ( J:90324 )
• multiple ureters arising from a single kidney are seen in all homozygous mutants
• all of the ureters remain connected to the nephric duct rather than undergoing remodeling to connect to the bladder
hydroureter ( J:90324 )
• at E18.5 the collecting ducts and ureters are grossly dilated
abnormal ureteric bud morphology ( J:90324 )
• at E11.5 multiple ureteric buds (3 or more buds in 14/16 mutants, and 2 in 2/16) emerge from the nephric duct rather than a single bud as in wild-type mice
• some of these buds project medially rather than dorsally

integument
abnormal mammary gland duct morphology ( J:106011 )
• end buds formed by transplanted mammary analage display enlarged subcapsular spaces, invaginated cap cell layers and disorganized luminal epithelial cells
• sporadic loss of luminal epithelial cells and separation of epithelial cell layers is detected in transplanted mammary glands

digestive/alimentary system
abnormal foregut morphology ( J:193400 )
• closer foregut-to-body-wall distance compared to controls at E10.5




Genotype
MGI:2682063
cn2
Allelic
Composition		 Ext1tm1Yama/Ext1+
Slit2tm1Matl/Slit2tm1Matl
Tg(Nes-cre)1Kln/0
Genetic
Background		 involves: 129S2/SvPas * 129S5/SvEvBrd * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ext1tm1Yama mutation (0 available); any Ext1 mutation (63 available)
Slit2tm1Matl mutation (2 available); any Slit2 mutation (88 available)
Tg(Nes-cre)1Kln mutation (4 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal axon guidance ( J:86465 )
• 59-67% of retinal axons project ectopically into the contralateral optic nerve

cellular
abnormal axon guidance ( J:86465 )
• 59-67% of retinal axons project ectopically into the contralateral optic nerve




Genotype
MGI:5522645
cx3
Allelic
Composition		 Ntn1Gt(ST629)Byg/Ntn1Gt(ST629)Byg
Slit2tm1Matl/Slit2tm1Matl
Genetic
Background		 involves: 129P2/OlaHsd * 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ntn1Gt(ST629)Byg mutation (1 available); any Ntn1 mutation (106 available)
Slit2tm1Matl mutation (2 available); any Slit2 mutation (88 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal cell adhesion ( J:106011 )
• severe defects in adhesion are seen in the mammary gland ducts
• in culture myoepithelial cells are impaired in their ability to adhere to luminal epithelial cells

endocrine/exocrine glands
abnormal mammary gland duct morphology ( J:106011 )
• end buds formed by transplanted mammary analage display subcapsular space, dissociated cap cells, cap cell layers devoid of underlying luminal epithelial cells, regions of disrupted cap cells, and regions of invaginated cap cell layers
• breaks in the basal lamina are seen and severe adhesion defects are detected
• between the luminal epithelial cells and the myoepithelial cells

integument
abnormal mammary gland duct morphology ( J:106011 )
• end buds formed by transplanted mammary analage display subcapsular space, dissociated cap cells, cap cell layers devoid of underlying luminal epithelial cells, regions of disrupted cap cells, and regions of invaginated cap cell layers
• breaks in the basal lamina are seen and severe adhesion defects are detected
• between the luminal epithelial cells and the myoepithelial cells




Genotype
MGI:3043188
cx4
Allelic
Composition		 Slit1tm1.1Matl/Slit1tm1.1Matl
Slit2tm1Matl/Slit2tm1Matl
Slit3tm1.1Dor/Slit3tm1.1Dor
Genetic
Background		 involves: 129S1/Sv * 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slit1tm1.1Matl mutation (0 available); any Slit1 mutation (70 available)
Slit2tm1Matl mutation (2 available); any Slit2 mutation (88 available)
Slit3tm1.1Dor mutation (1 available); any Slit3 mutation (80 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal axon guidance ( J:89780 )
• at E12.5 commissural axons appear disorganized as they exit the floor plate lacking the typical bundled morphology
• at E12.5 more commissural axons stall and fail to exit the floor plate (78% versus 28% in controls) or recross the floor plate (~20% versus 0% in controls; controls in this study were Slit1tm1.1Matl homozygotes)
abnormal spinal cord lateral column morphology ( J:89780 )
• the lateral funiculus (white matter tract) is significantly thinner in triple homozygous mutants compared to controls

cellular
abnormal axon guidance ( J:89780 )
• at E12.5 commissural axons appear disorganized as they exit the floor plate lacking the typical bundled morphology
• at E12.5 more commissural axons stall and fail to exit the floor plate (78% versus 28% in controls) or recross the floor plate (~20% versus 0% in controls; controls in this study were Slit1tm1.1Matl homozygotes)




Genotype
MGI:5522767
cx5
Allelic
Composition		 Slit2tm1Matl/Slit2tm1Matl
Slit3tm1.1Dor/Slit3tm1.1Dor
Genetic
Background		 involves: 129S1/Sv * 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slit2tm1Matl mutation (2 available); any Slit2 mutation (88 available)
Slit3tm1.1Dor mutation (1 available); any Slit3 mutation (80 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
increased mammary gland epithelial cell proliferation ( J:141875 )
• in mammary gland ducts formed by transplanted mammary anlage

endocrine/exocrine glands
increased mammary gland epithelial cell proliferation ( J:141875 )
• in mammary gland ducts formed by transplanted mammary anlage
abnormal mammary gland duct morphology ( J:141875 )
• mammary gland ducts formed by transplanted mammary anlage display hyperplasticity and organization defects, including mild to severe defects in ductal lumens and disrupted growth control, 100% penetrant phenotype

integument
increased mammary gland epithelial cell proliferation ( J:141875 )
• in mammary gland ducts formed by transplanted mammary anlage
abnormal mammary gland duct morphology ( J:141875 )
• mammary gland ducts formed by transplanted mammary anlage display hyperplasticity and organization defects, including mild to severe defects in ductal lumens and disrupted growth control, 100% penetrant phenotype




Genotype
MGI:3043191
cx6
Allelic
Composition		 Slit1tm1.1Matl/Slit1tm1.1Matl
Slit2tm1Matl/Slit2tm1Matl
Slit3tm1.1Dor/Slit3+
Genetic
Background		 involves: 129S1/Sv * 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slit1tm1.1Matl mutation (0 available); any Slit1 mutation (70 available)
Slit2tm1Matl mutation (2 available); any Slit2 mutation (88 available)
Slit3tm1.1Dor mutation (1 available); any Slit3 mutation (80 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal axon guidance ( J:89780 )
• at E12.5 commissural axons occasionally recross the floor plate, this is never seen in controls
abnormal spinal cord ventral commissure morphology ( J:89780 )
• the ventral commissure (bundle of nerve fibers passing from one side to the other in the spinal cord) appears thicker compared to controls
abnormal spinal cord lateral column morphology ( J:89780 )
• the ventral funiculus (white matter tract) appears reduced in mutants compared to controls

cellular
abnormal axon guidance ( J:89780 )
• at E12.5 commissural axons occasionally recross the floor plate, this is never seen in controls




Genotype
MGI:3043170
cx7
Allelic
Composition		 Gdnftm1Rosl/Gdnf+
Slit2tm1Matl/Slit2tm1Matl
Genetic
Background		 involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gdnftm1Rosl mutation (0 available); any Gdnf mutation (18 available)
Slit2tm1Matl mutation (2 available); any Slit2 mutation (88 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
renal/urinary system
abnormal ureter morphology ( J:90324 )
• at E14.5 in 6/30 kidneys 2 ureters or 1 ureter and a blind-ending ectopic protusion are found, in 24/30 only a single ureter is found demonstrating a partial rescue of the Slit2tm1Matl homozygous phenotype
• in 5/15 kidneys with a single ureter, the single ureter did not undergo remodeling and remained connected to the nephric duct, in the other 10/15 ureter remodeling was also rescued with the ureter connected to the bladder




Genotype
MGI:5522757
cx8
Allelic
Composition		 Slit1tm1.1Matl/Slit1tm1.1Matl
Slit2tm1Matl/Slit2+
Genetic
Background		 involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slit1tm1.1Matl mutation (0 available); any Slit1 mutation (70 available)
Slit2tm1Matl mutation (2 available); any Slit2 mutation (88 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal olfactory tract morphology ( J:119469 )
• thinner and beginning to divide into bundles at E14.5




Genotype
MGI:5522762
cx9
Allelic
Composition		 Slit1tm1.1Matl/Slit1+
Slit2tm1Matl/Slit2tm1Matl
Genetic
Background		 involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slit1tm1.1Matl mutation (0 available); any Slit1 mutation (70 available)
Slit2tm1Matl mutation (2 available); any Slit2 mutation (88 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal olfactory tract morphology ( J:119469 )
• some small bundles diverge from the main tract and extend ventrally, at E18.5




Genotype
MGI:3772917
cx10
Allelic
Composition		 Slit1tm1.1Matl/Slit1tm1.1Matl
Slit2tm1Matl/Slit2tm1Matl
Genetic
Background		 involves: 129S2/SvPas
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slit1tm1.1Matl mutation (0 available); any Slit1 mutation (70 available)
Slit2tm1Matl mutation (2 available); any Slit2 mutation (88 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging

nervous system
nervous system phenotype ( J:130430 )
N
• mice exhibit normal numbers of interneurons during development
abnormal olfactory lobe morphology ( J:119469 )
• density of LOT (guidepost) cells is slightly decreased rostrally near the ventral part of the olfactory bulb
abnormal olfactory tract morphology ( J:119469 )
• highly defasciculated into small axonal bundles fanned all over the ventral side of the telencephalon although a subset of axons continue on the normal trajectory, at E18.5
• medial axons are more severely affected than lateral axons
abnormal embryonic/fetal subventricular zone morphology ( J:130430 )
• at E13.5 and E16.5, cell proliferation of ganglionic eminences in the subventricular zone is increased compared to in Slit1tm1.1Matl homozygotes
abnormal neurite morphology ( J:130430 )
• in the subventricular zone/intermediate zone and subplate, mic exhibit an increased in neurite process length is, the number of neurites, and the degree of branching




Genotype
MGI:3043203
cx11
Allelic
Composition		 Robo3tm1Matl/Robo3tm1Matl
Slit1tm1.1Matl/Slit1tm1.1Matl
Slit2tm1Matl/Slit2tm1Matl
Genetic
Background		 involves: 129/Sv * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo3tm1Matl mutation (1 available); any Robo3 mutation (71 available)
Slit1tm1.1Matl mutation (0 available); any Slit1 mutation (70 available)
Slit2tm1Matl mutation (2 available); any Slit2 mutation (88 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal axon guidance ( J:89438 )
• embryos show some midline crossing although the majority still fail to cross indicating a partial rescue of the Robo3tm1Matl homozygous phenotype

cellular
abnormal axon guidance ( J:89438 )
• embryos show some midline crossing although the majority still fail to cross indicating a partial rescue of the Robo3tm1Matl homozygous phenotype




Genotype
MGI:3043199
cx12
Allelic
Composition		 Robo3tm1Matl/Robo3tm1Matl
Slit2tm1Matl/Slit2tm1Matl
Genetic
Background		 involves: 129/Sv * CD-1
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Robo3tm1Matl mutation (1 available); any Robo3 mutation (71 available)
Slit2tm1Matl mutation (2 available); any Slit2 mutation (88 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
nervous system
abnormal axon guidance ( J:89438 )
• embryos show some midline crossing although the majority still fail to cross indicating a partial rescue of the Robo3tm1Matl homozygous phenotype

cellular
abnormal axon guidance ( J:89438 )
• embryos show some midline crossing although the majority still fail to cross indicating a partial rescue of the Robo3tm1Matl homozygous phenotype




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Send questions and comments to User Support. 		last database update
04/30/2024
MGI 6.23 		The Jackson Laboratory