Phenotypes associated with this allele
Allelic Composition |
Scxtm1Eno/Scxtm2Eno
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Genetic Background |
chimera involves: 129S7/SvEvBrd * C57BL/6 * Swiss |
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Scxtm1Eno mutation
(0 available);
any
Scx mutation
(12 available)
Scxtm2Eno mutation
(0 available);
any
Scx mutation
(12 available)
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embryo
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• in two E9.5 chimeric embryos with >95% mutant cells, discernable somites are absent and the somitic region is cell-deficient
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cardiovascular system
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• n two E9.5 chimeric embryos with >95% mutant cells, the dorsal aortae become dilated to fill the space created by the complete loss of identifiable somites
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skeleton
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• in chimeric embryos, mutant cells are unable to contribute to (i) the sclerotomal compartment of somites that gives rise to the axial skeleton at E8.5 and E9.5 and (ii) the developing ribs at E14.5 and E16.5
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Allelic Composition |
Scxtm1Eno/Scxtm1Eno Gt(ROSA)26Sortm1Sor/Gt(ROSA)26Sor+
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Genetic Background |
either: (involves: 129S4/SvJaeSor * 129S7/SvEvBrd * Swiss) or (involves: 129S4/SvJaeSor * 129S7/SvEvBrd * C57BL/6) |
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Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Gt(ROSA)26Sortm1Sor mutation
(8 available);
any
Gt(ROSA)26Sor mutation
(944 available)
Scxtm1Eno mutation
(0 available);
any
Scx mutation
(12 available)
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mortality/aging
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• by E8.5, homozygotes appear to resorb or degenerate and lack distinct features; no mutants are detected after E8.5
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embryo
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• mutants are unable to show even the earliest signs of gastrulation
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• homozygotes appear normal in size and morphology at the early egg cylinder stage (E6.0), but fail to develop beyond this stage
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• at E6.5 and E7.5, homozygotes exhibit growth retardation relative to wild-type embryos
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• up to E6.0, mutant embryos appear normal and contain embryonic and extraembryonic structures and proamniotic cavities; however, by E6.5, the simple egg cylinder fails to elongate
• homozygotes show a rapid decline of proliferative activity and elevated cell death in the epiblast at the time of egg cylinder elongation; in contrast, proliferation occurs normally prior to E6.0
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• mutant embryos fail to form a histologically recognizable mesoderm at gastrulation; they fail to form mesodermal cells or express mesodermal markers
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• mutant embryos contain abnormal pyknotic cells and fail to form a primitive streak
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• by E6.5, mutant extraembryonic regions appear to be underdeveloped relative to wild-type; a cavitated extraembryonic region fails to form
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• mutant ectoplacental cones are proportionately correct in size but degenerate after E6.5
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• visceral and parietal endoderm layers are present at E6.5 and E7.5, but appear developmentally delayed and fail to form the flattened endodermal cell phenotype in the embryonic region
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• visceral and parietal endoderm layers are present at E6.5 and E7.5, but appear developmentally delayed and fail to form the flattened endodermal cell phenotype in the embryonic region
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growth/size/body
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• at E6.5 and E7.5, homozygotes exhibit growth retardation relative to wild-type embryos
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