Phenotypes associated with this allele
Allelic
Composition |
Pax5tm1Mbu/Pax5tm1Mbu
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Genetic
Background |
either: 129 or (involves: 129S2/SvPas * C57BL/6J) |
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax5tm1Mbu mutation
(1 available);
any
Pax5 mutation
(35 available)
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hematopoietic system
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• no pre-B cell lines can be obtained from Pax5-deficient livers while they are readily established from livers of fetal heterozygous and wild-type livers
• fetal liver cannot support development of early B-lymphoid progenitors
• when fetal liver cells from mutants are transplanted into lethally irradiated hosts however, pre-B cell precursors can be detected in bone marrow of hosts
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• incidence of V to DJ rearrangement of the immunoglobulin heavy chain is reduced 10-fold in Pax5-deficient mice
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• development is halted at early pro-B cell stage
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• B cell development is arrested at the early pro-B stage in bone marrow
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immune system
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• no pre-B cell lines can be obtained from Pax5-deficient livers while they are readily established from livers of fetal heterozygous and wild-type livers
• fetal liver cannot support development of early B-lymphoid progenitors
• when fetal liver cells from mutants are transplanted into lethally irradiated hosts however, pre-B cell precursors can be detected in bone marrow of hosts
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• incidence of V to DJ rearrangement of the immunoglobulin heavy chain is reduced 10-fold in Pax5-deficient mice
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• development is halted at early pro-B cell stage
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• B cell development is arrested at the early pro-B stage in bone marrow
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax5tm1Mbu mutation
(1 available);
any
Pax5 mutation
(35 available)
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mortality/aging
immune system
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• B cell development is arrested in the pro-B cell stage
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• in a transwell migration assay, pro-B cells have a 4-fold increase in migration towards a source of CXCL12 compared to wild-type mice
• in culture pro-B cells exhibit 32 times poorer adhesion to a VCAM-1 coated surface compared to wild-type cells
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growth/size/body
cellular
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• in culture pro-B cells exhibit 32 times poorer adhesion to a VCAM-1 coated surface compared to wild-type cells
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• in a transwell migration assay, pro-B cells have a 4-fold increase in migration towards a source of CXCL12 compared to wild-type mice
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hematopoietic system
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• B cell development is arrested in the pro-B cell stage
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• in a transwell migration assay, pro-B cells have a 4-fold increase in migration towards a source of CXCL12 compared to wild-type mice
• in culture pro-B cells exhibit 32 times poorer adhesion to a VCAM-1 coated surface compared to wild-type cells
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax5tm1Mbu mutation
(1 available);
any
Pax5 mutation
(35 available)
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mortality/aging
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• only 11/200 animals survive past weaning and live up to 7 months
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• homozygotes usually die in third week after birth
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growth/size/body
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• mice show severe growth retardation in the second week after birth; mice that survive through weaning are severely runted
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• at time of death, weight is ~1/3 weight of littermates
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behavior/neurological
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• mice consistently flex both hindlimbs and sometimes their forelimbs upon tail suspension, while wild-type mice extend their legs
• behavior is pronounced in young animals 2-3 weeks of age, but becomes attenuated in adults which usually clasp only 1 hindleg
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nervous system
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• at E16.5 posterior region of the collicular neuroepithelium is detectably shorter and thinner vs wild-type
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• mice show altered cerebellar foliation pattern
• lobule culmen is divided by a deep intraculminate fissure
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• structure is dramatically reduced in central region around midline while appearing normal on lateral sides
• however, superior colliculus is of normal size
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• culmen of cerebellum is expanded anteriorly
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• minor change in folding of tuber vermis is observed
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immune system
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• fetal liver does not support B cell lymphopoiesis; wild-type and heterozygous livers contain only 3-4% B lymphocytes in total population but mutants have no B lymphocytes at E17
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• incidence of V to DJ heavy chain rearrangements is 50- to 70-fold reduced in mutant pre-BI cells compared to wild-type; D to J rearrangements are not affected
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• at 2 weeks of age, numbers of immature B cells in spleen are considerably reduced; similar results are found in bone marrow and lymph nodes
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• B cell development is stopped at early stage; B cells are large and express CD43 and B220 on the surface consistent with early precursor stage
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• mutants lack mature B cells
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• at 2 weeks, peritoneum is devoid of conventional B cells and mature B-1 cells
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• at 2 weeks, peritoneum is devoid of conventional B cells
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• mature T cells are present in spleen
• number of mature T cells appears increased 2- to 4-fold as result of deletion of mature B cells
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• no immunoglobulin is detected above detection limit compared to normal concentrations in wild-type and heterozygotes
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hematopoietic system
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• fetal liver does not support B cell lymphopoiesis; wild-type and heterozygous livers contain only 3-4% B lymphocytes in total population but mutants have no B lymphocytes at E17
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• incidence of V to DJ heavy chain rearrangements is 50- to 70-fold reduced in mutant pre-BI cells compared to wild-type; D to J rearrangements are not affected
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• at 2 weeks of age, numbers of immature B cells in spleen are considerably reduced; similar results are found in bone marrow and lymph nodes
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• B cell development is stopped at early stage; B cells are large and express CD43 and B220 on the surface consistent with early precursor stage
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• mutants lack mature B cells
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• at 2 weeks, peritoneum is devoid of conventional B cells and mature B-1 cells
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• at 2 weeks, peritoneum is devoid of conventional B cells
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• mature T cells are present in spleen
• number of mature T cells appears increased 2- to 4-fold as result of deletion of mature B cells
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• no immunoglobulin is detected above detection limit compared to normal concentrations in wild-type and heterozygotes
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hearing/vestibular/ear
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N |
• at 13-19 days of age, homozygotes exhibit normal auditory responses and audiograms relative to wild-type mice, suggesting that the affected central region of the inferior colliculus is not important for hearing, at least as assessed by early brainstem auditory evoked potential (BAEP) measurements
• however, development of auditory sensitivity is delayed by at least 1 day, and peak latencies of BAEPs for waves II and IV are significantly longer, consistent with a general growth retardation
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Allelic
Composition |
Pax5tm1Mbu/Pax5+
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Genetic
Background |
involves: 129S2/SvPas * C57BL/6 |
|
| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Pax5tm1Mbu mutation
(1 available);
any
Pax5 mutation
(35 available)
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nervous system
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• culmen of cerebellum is slightly expanded anteriorly, but inferior colliculus appears normal as opposed to homozygotes
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• only shallow intraculminate fissure is observed compared to homozygotes
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immune system
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• LPS-stimulated lymph node B cells exhibit a low frequency of and a delay in increased cell size relative to wild-type cells
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• IgG class switching is decreased
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• short-lived IgM-B220lo and IgM+B220lo immature B cells are slightly lower in number
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• there is a 3-fold reduction in the number of long-lived mature B cells
• mice lack recirculating IgM+IgD+B220hi cells in the bone marrow
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• short-lived IgM-B220lo B cells are slightly lower in number
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• total immunoglobin is reduced 3-fold compared to control mice
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• IgG levels are decreased ranging from a 9.4-fold decrease of IgG1 to a 2-fold decrease in IgG3 levels
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hematopoietic system
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• LPS-stimulated lymph node B cells exhibit a low frequency of and a delay in increased cell size relative to wild-type cells
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• IgG class switching is decreased
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• short-lived IgM-B220lo and IgM+B220lo immature B cells are slightly lower in number
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• there is a 3-fold reduction in the number of long-lived mature B cells
• mice lack recirculating IgM+IgD+B220hi cells in the bone marrow
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• short-lived IgM-B220lo B cells are slightly lower in number
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• total immunoglobin is reduced 3-fold compared to control mice
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• IgG levels are decreased ranging from a 9.4-fold decrease of IgG1 to a 2-fold decrease in IgG3 levels
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immune system
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• half of the mature B cells in the spleen are absent
• only a small number of mature B cells are lost upon short-term inactivation of Pax5
• polyinosine-polycystosine (pIpC)-treated mice lack IgD+B220hi cells
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hematopoietic system
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• half of the mature B cells in the spleen are absent
• only a small number of mature B cells are lost upon short-term inactivation of Pax5
• polyinosine-polycystosine (pIpC)-treated mice lack IgD+B220hi cells
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| Find Mice |
Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ightm1(PAX5)Mbu mutation
(0 available);
any
Igh mutation
(43 available)
Pax5tm1Mbu mutation
(1 available);
any
Pax5 mutation
(35 available)
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hematopoietic system
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• mice have partial block in development at pro-B cell to pre-B cells transition in bone marrow
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• splenic B cell numbers were decreased by 6-fold compared to wild-type
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immune system
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N |
• early B cell block seen in Pax5-deficient mice is rescued, as shown by presence of IgM+IgD+ mature B cells
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• mice have partial block in development at pro-B cell to pre-B cells transition in bone marrow
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• splenic B cell numbers were decreased by 6-fold compared to wild-type
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