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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Il4tm1Nnt
targeted mutation 1, Nancy Noben-Trauth
MGI:1857196
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Il4tm1Nnt/Il4tm1Nnt C57BL/6-Il4tm1Nnt/J MGI:3580392
hm2
Il4tm1Nnt/Il4tm1Nnt involves: C57BL/6 MGI:3850555
cx3
Fbn1Tsk/Fbn1+
Il4tm1Nnt/Il4tm1Nnt
involves: C57BL/6 * C57BL/10 * DBA/2 MGI:5301103


Genotype
MGI:3580392
hm1
Allelic
Composition
Il4tm1Nnt/Il4tm1Nnt
Genetic
Background
C57BL/6-Il4tm1Nnt/J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il4tm1Nnt mutation (1 available); any Il4 mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• 5 weeks after Helicobacter pylori infection, mice have a significantly increased gastritis inflammation score compared to infected wild-type mice
• microglial cells fail to express the cell marker Ym1 (Chi3l3)
• there is enhanced IgA production by lymphocytes isolated from either the spleen or liver granulomas after schistosome infection
• lymphocytes from the spleen or from liver granulomas fail to secrete IgE in response to Schistosoma mansoni infection
• serum IgG1 levels are dramatically decreased in response to schistosome infection compared to the wild-type response
• isolated lymphocytes from the spleen or from liver granulomas produce very little IgG1 in culture
• very little IgM is produced by lymphocytes that are isolated from liver granulomas
• lymphocytes isolated from liver granulomas resulting from schistosome infection lack a strong Th2 response
• the lack of Th2 response is reflected in no secretion of IL-4, IL-5, and a 50% reduction in IL-10 by lymphocytes isolated from liver granulomas
• liver granuloma lymphocytes make small amounts of IFN-gamma compared to wild-type lymphocytes that make no IFN-gamma
• lymphocytes isolated from liver granulomas produce dectectable amounts of IFN-gamma compared to wild-type lymphocytes that produce no IFN-gamma (J:37196)
• splenocytes from Helicobacter pylori-infected mice produce log fold higher amounts of IFN-gamma than splenocytes from wild-type controls when cultured in the presence of H. pylori antigen (J:120556)
• activated splenic lymphocytes fail to secrete IL-10 (J:37196)
• IL-10 secretion by lymphocytes isolated from liver granulomas is also severely impaired (J:37196)
• splenocytes from Helicobacter pylori infected mice produce 4-fold less IL-10 when cultured in the presence of H. pylori antigen (J:120556)
• lymphocytes make no IL-4
• secretion of IL-5 is almost absent by unactivated lymphocytes isolated from liver granulomas
• activated lymphocytes from liver granulomas produced 50% IL-5 compared to controls
• secretion of IL-5 by activated splenic lymphocytes is also severely impaired
• mice generate 32% smaller liver granulomas than wild-type mice in response to schistosome ova 8 weeks after infection
• the granulomas contain few eosinophils and no mast cells compared to their wild-type counterparts
• granulomas contain2-fold more lymphocytes and 42% more macrophages than in wild-type mice
• the number of B cells in the liver granulomas increases more than 2-fold while the number of CD4 T cells decreases by almost the same amount
• B cells in granulomas have lower surface expression of Class II and IgE receptor
• lethally irradiated mutant mice can be transplanted with bone marrow from wild-type mice and then have encephalomyelitis induced by injection of an inflammatory antigen
• these bone marrow chimera mice exhibit more severe inflammation with a 2-fold increase in the number of infiltrating lymphocytes, higher disease score, and earlier onset of disease
• the number of infiltrating macrophages and activated resident microglial cells are also increased in these bone marrow chimeras as a result of encephalomyelitis induction

digestive/alimentary system
• 5 weeks after Helicobacter pylori infection, mice have a significantly increased gastritis inflammation score compared to infected wild-type mice

hematopoietic system
• microglial cells fail to express the cell marker Ym1 (Chi3l3)
• there is enhanced IgA production by lymphocytes isolated from either the spleen or liver granulomas after schistosome infection
• lymphocytes from the spleen or from liver granulomas fail to secrete IgE in response to Schistosoma mansoni infection
• serum IgG1 levels are dramatically decreased in response to schistosome infection compared to the wild-type response
• isolated lymphocytes from the spleen or from liver granulomas produce very little IgG1 in culture
• very little IgM is produced by lymphocytes that are isolated from liver granulomas
• lymphocytes isolated from liver granulomas resulting from schistosome infection lack a strong Th2 response
• the lack of Th2 response is reflected in no secretion of IL-4, IL-5, and a 50% reduction in IL-10 by lymphocytes isolated from liver granulomas
• liver granuloma lymphocytes make small amounts of IFN-gamma compared to wild-type lymphocytes that make no IFN-gamma

nervous system
• microglial cells fail to express the cell marker Ym1 (Chi3l3)
• lethally irradiated mutant mice can be transplanted with bone marrow from wild-type mice and then have encephalomyelitis induced by injection of an inflammatory antigen
• these bone marrow chimera mice exhibit more severe inflammation with a 2-fold increase in the number of infiltrating lymphocytes, higher disease score, and earlier onset of disease
• the number of infiltrating macrophages and activated resident microglial cells are also increased in these bone marrow chimeras as a result of encephalomyelitis induction




Genotype
MGI:3850555
hm2
Allelic
Composition
Il4tm1Nnt/Il4tm1Nnt
Genetic
Background
involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il4tm1Nnt mutation (1 available); any Il4 mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• lymphocytes fail to produce IL-4
• some mice have a less severe degree of airway hyperresponsiveness after sensitization and challenge with OVA peptide




Genotype
MGI:5301103
cx3
Allelic
Composition
Fbn1Tsk/Fbn1+
Il4tm1Nnt/Il4tm1Nnt
Genetic
Background
involves: C57BL/6 * C57BL/10 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fbn1Tsk mutation (2 available); any Fbn1 mutation (110 available)
Il4tm1Nnt mutation (1 available); any Il4 mutation (33 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• no Il4 is produced by Th2 cells

integument
N
• no skin fibrosis develops

respiratory system
• emphysema develops as expected for heterozygous Fbn1Tsk





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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
01/18/2022
MGI 6.17
The Jackson Laboratory