Rr29^em2Andg
Endonuclease-mediated Allele Detail

Summary

• Symbol: Rr29^em2Andg
• Name: regulatory region 29; endonuclease-mediated mutation 2, Guillaume Andrey
• MGI ID: MGI:7703771
• Synonyms: deltaCTCF ZRS
• Gene: Rr29 Location: Chr5:29519495-29520860 bp, + strand
• Alliance: Rr29^em2Andg page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:276559
• Parent Cell Line: G4 (ES Cell)
• Strain of Origin: (129S6/SvEvTac x C57BL/6NCrl)F1

Mutation description

• Allele Type: Endonuclease-mediated (Modified regulatory region)
• Mutation: Intragenic deletion
• Mutation details: A CTCF-binding site associated with Shh enhancer ZRS or MFCS1, located in Lmbr1 intron 5, was deleted using an sgRNA with CRISPR/Cas9 technology in ESCs that carry the Rr112510^em2Andg and Rr112507^em1Andg CTCF-binding site deletion alleles. This allele represents two different deletions (ACCAGTGCTCCCTAGTGGGGG on one copy of chromosome 5 and CTCCCTAGTGGGGGAGAG on the other) that overlap the binding site TGCTCCCTAGTGGGGGAGA. (J:276559)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr29 Mutation: 2 strains or lines available

References

• Original: J:276559 Paliou C, et al., Preformed chromatin topology assists transcriptional robustness of Shh during limb development. Proc Natl Acad Sci U S A. 2019 Jun 18;116(25):12390-12399
• All: 2 reference(s)