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Rr112507em1Andg
Endonuclease-mediated Allele Detail
Summary
Symbol: Rr112507em1Andg
Name: regulatory region 112507; endonuclease-mediated mutation 1, Guillaume Andrey
MGI ID: MGI:7703762
Synonyms: deltaCTCF i5
Gene: Rr112507  Location: Chr5:29498201-29498800 bp  Genetic Position: Chr5, Syntenic
Alliance: Rr112507em1Andg page
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:276559
Parent Cell Line:  G4 (ES Cell)
Strain of Origin:  (129S6/SvEvTac x C57BL/6NCrl)F1
Mutation
description
Allele Type:    Endonuclease-mediated (Modified regulatory region)
Mutation:    Intragenic deletion
 
Mutation detailsThe CTCF-binding site, located in Lmbr1 intron 5, was deleted using an sgRNA with CRISPR/Cas9 technology. This allele represents two different deletions (CTAGTGGCCAA on one copy of chromosome 5 and CTGGAGTCCTCTAGTGGCCAACTGGAGAA on the other) that overlap/contain the binding site AGTCCTCTAGTGGCCAACT. (J:276559)
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rr112507 Mutation:  0 strains or lines available
References
Original:  J:276559 Paliou C, et al., Preformed chromatin topology assists transcriptional robustness of Shh during limb development. Proc Natl Acad Sci U S A. 2019 Jun 18;116(25):12390-12399
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory