Igkj5^VJkappa5Durw
Allele Detail

Summary

• Symbol: Igkj5^VJkappa5Durw
• Name: immunoglobulin kappa joining 5; VJkappa5, Duane R Wesemann
• MGI ID: MGI:6195826
• Gene: Igkj5 Location: Chr6:70700870-70700907 bp, + strand Genetic Position: Chr6, 32.04 cM
• Alliance: Igkj5^VJkappa5Durw page

Mutation origin

• Strain of Origin: (C57BL/6 x 129S4/SvJae)F1

Mutation description

• Allele Type: Other (Not Applicable)
• Mutation: Other
• Mutation details: A mature B cell, where the the immunoglobulin kappa chain complex had undergone Igk-V to Igkj5 recombination, was isolated and reprogrammed to an iPSC (induced pluripotent stem cell). This iPSC was then used to create a homozygous mouse line where all cells contain this allele. The mouse from which the B cells were isolated is a reprogrammable mouse containing the Col1a1^{tm1(tetO-Pou5f1,-Klf4,-Sox2,-Myc)Hoch} and Gt(ROSA)26Sor^{tm1(rtTA*M2)Jae} alleles which allow for the reprogramming to be induced by doxycycline. (J:252910)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Igkj5 Mutation: 0 strains or lines available

References

• Original: J:252910 Tong P, et al., IgH isotype-specific B cell receptor expression influences B cell fate. Proc Natl Acad Sci U S A. 2017 Oct 3;114(40):E8411-E8420
• All: 1 reference(s)