Piezo1^{tm1c(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Piezo1^{tm1c(KOMP)Wtsi}
• Name: piezo-type mechanosensitive ion channel component 1; targeted mutation 1c, Wellcome Trust Sanger Institute
• MGI ID: MGI:5823989
• Gene: Piezo1 Location: Chr8:123208437-123278068 bp, - strand Genetic Position: Chr8, 71.31 cM
• Alliance: Piezo1^{tm1c(KOMP)Wtsi} page
• IMPC: Piezo1 gene page

Mutation origin

• Mutant Cell Line: EPD0500_5_F12
• Germline Transmission: Earliest citation of germline transmission: J:217082
• Parent Cell Line: JM8A3.N1 (ES Cell)
• Strain of Origin: C57BL/6N-A^tm1Brd
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Conditional ready)
• Mutation: Insertion Vector: L1L2_Bact_P
• Mutation details: The L1L2_Bact_P cassette was inserted at position 123219179 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 123222950. A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving the critical exon(s) flanked by loxP sites. (J:217082)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Piezo1 Mutation: 87 strains or lines available

References

• Original: J:217082 Li J, et al., Piezo1 integration of vascular architecture with physiological force. Nature. 2014 Nov 13;515(7526):279-82
• All: 12 reference(s)