Kash5^{tm1b(KOMP)Wtsi}
Targeted Allele Detail

Summary

• Symbol: Kash5^{tm1b(KOMP)Wtsi}
• Name: KASH domain containing 5; targeted mutation 1b, Wellcome Trust Sanger Institute
• MGI ID: MGI:5700363
• Synonyms: Kash5-null
• Gene: Kash5 Location: Chr7:44833048-44854316 bp, - strand Genetic Position: Chr7, 29.17 cM
• Alliance: Kash5^{tm1b(KOMP)Wtsi} page
• IMPC: Kash5 gene page

Small testes and narrow seminiferous tubules devloid of elongated spermatids and mature sperm in Kash5^{tm1b(KOMP)Wtsi}/Kash5^{tm1b(KOMP)Wtsi} males

Show the 6 phenotype image(s) involving this allele.

Mutation origin

• Mutant Cell Line: EPD0094_2_G08
• Germline Transmission: Earliest citation of germline transmission: J:201813
• Parent Cell Line: JM8.N4 (ES Cell)
• Strain of Origin: C57BL/6N
• Project Collection: KOMP-CSD

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Intragenic deletion Vector: L1L2_Bact_P
• Mutation details: Mice carrying the Ccdc155^{tm1a(KOMP)Wtsi} conditional allele were crossed with C57/B6 animals harboring a Tg(Zp3-cre)93Knw transgene to delete exons 5-8, resulting in a frame shift and the appearance of a stop codon 36 bp downstream of the 3' end of exon 4. Mice carrying this recombined allele were bred to remove Cre and were maintained as heterozygotes. Quantitative RT-PCR and Western blot analyses of whole testis lysates confirmed the complete absence of both mRNA and protein from homozygous mutant males. Testis samples from heterozygous males showed an intermediate (~50%) level of mRNA and protein. (J:201813)

Expression

In Structures Affected by this Mutation:

6 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Kash5 Mutation: 17 strains or lines available

References

• Original: J:201813 Horn HF, et al., A mammalian KASH domain protein coupling meiotic chromosomes to the cytoskeleton. J Cell Biol. 2013 Sep 30;202(7):1023-39
• All: 2 reference(s)