Del(16Dyrk1a-Kcnj6)14Yey
Targeted Allele Detail

Summary

• Symbol: Del(16Dyrk1a-Kcnj6)14Yey
• Name: deletion, Chr 16, Y Eugene Yu 14
• MGI ID: MGI:5689441
• Synonyms: Df(16)7, Df(16)7Yey
• Gene: Del(16Dyrk1a-Kcnj6)14Yey Location: unknown Genetic Position: Chr16, Syntenic

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:224899
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intergenic deletion, Intragenic deletion
• Del(16Dyrk1a-Kcnj6)14Yey involves 2 genes/genome features (Dyrk1a, Kcnj6) View all
• Mutation details: Chromosome-engineering cassettes were inserted into mouse Chromosome 16 to bracket a span between the dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 1a (Dyrk1a) gene and the potassium inwardly-rectifying channel, subfamily J, member 6 (Kcnj6) gene. The cassette placed at the proximal locus (MICER clone MHPN85f19) was targeted upstream of Dyrk1a and contained a neomycin resistance gene, a loxP site, a 5' portion of an HPRT minigene, and a tyrosinase minigene. The cassette placed at the distal locus (MICER clone MHPP54c08) was targeted downstream of the Kcnj6 gene and contained an agouti transgene, a 3' portion of an HPRT minigene, a loxP site and puromycin resistance gene. Double-targeted ES cells were transiently transfected with the cre recombinase expression vector pOG231 to remove Dyrk1a and Kcnj6. FISH analysis of ES cell clones confirmed the deletions. (J:224899)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Del(16Dyrk1a-Kcnj6)14Yey Mutation: 1 strain or line available

References

• Original: J:224899 Jiang X, et al., Genetic dissection of the Down syndrome critical region. Hum Mol Genet. 2015 Nov 15;24(22):6540-51
• All: 1 reference(s)