Rr29^tm2Bobh
Targeted Allele Detail

Summary

• Symbol: Rr29^tm2Bobh
• Name: regulatory region 29; targeted mutation 2, Robert E Hill
• MGI ID: MGI:5613149
• Synonyms: ZRS^ETS+LACZ
• Gene: Rr29 Location: Chr5:29519495-29520860 bp, + strand
• Alliance: Rr29^tm2Bobh page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:208849
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Modified regulatory region)
• Mutations: Insertion, Intragenic deletion
• Rr29^tm2Bobh involves 1 genes/genome features (Lmbr1) View all
• Mutation details: A loxP site flanked lacZ and neomycin resistance gene cassette was inserted downstream of the Shh enhancer in intron 5 of the Lmbr1 gene and the 3' end of the region including all but the first ETS binding site was deleted via homologous recombination. The one remaining ETS1/GABPA binding site was mutated to prevent binding of ETS1/GABPA . (J:208849)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rr29 Mutation: 2 strains or lines available

References

• Original: J:208849 Lettice LA, et al., Development of five digits is controlled by a bipartite long-range cis-regulator. Development. 2014 Apr;141(8):1715-25
• All: 1 reference(s)