Gt(ROSA)26Sor^tm1Mgn
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^tm1Mgn
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Mark A Magnuson
• MGI ID: MGI:5085305
• Synonyms: ROSA26^LCA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM

Mutation origin

• Germline Transmission: Unknown
• Parent Cell Line: TL1/TL-1 (ES Cell)
• Strain of Origin: 129S6/SvEvTac

Mutation description

• Allele Type: Targeted
• Mutation: Insertion
• Gt(ROSA)26Sor^tm1Mgn involves 1 genes/genome features (Setd5) View all
• Mutation details: A ~5-kb segment of the gene including the promoter and first exon has been replaced by a lox71/lox2272-flanked pair of selection cassettes encoding a puromycin N-acetyltransferase/truncated HSV1 thymidine kinase fusion protein and neomycin phosphotransferae, respectively expressed under control of the mouse phosphoglycerate kinase promoter and the synthetic bacterial EM7 promoter (Pgk-puro/tk and EM7-neo). (J:174255)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

Notes

This allele was used to generate: Gt(ROSA)26Sor^tm12.1Mgn, Gt(ROSA)26Sor^{tm2.1(mix1b-mCherry)Mgn}, Gt(ROSA)26Sor^tm2Mgn, Gt(ROSA)26Sor^{tm3.1(RARE-CFP)Mgn}, Gt(ROSA)26Sor^tm3(CFP)Mgn, Gt(ROSA)26Sor^{tm4.1(RARE-YFP)Mgn}, Gt(ROSA)26Sor^tm4Mgn, Gt(ROSA)26Sor^{tm5.1(Smad7-CFP)Mgn}, Gt(ROSA)26Sor^{tm6.1(Smad7-CFP)Mgn}, Gt(ROSA)26Sor^{tm7.1(mix1b-mCherry)Mgn}, Gt(ROSA)26Sor^{tm8.1(Ctnnb1-CFP)Mgn}

References

• Original: J:174255 Chen SX, et al., Quantification of factors influencing fluorescent protein expression using RMCE to generate an allelic series in the ROSA26 locus in mice. Dis Model Mech. 2011 Jul-Aug;4(4):537-47
• All: 3 reference(s)