Summary | Mutation origin | Mutation description | Find Mice (IMSR) | Notes | References

Symbol:	Gt(ROSA)26Sortm5.1(Smad7-CFP)Mgn
Name:	gene trap ROSA 26, Philippe Soriano; targeted mutation 5.1, Mark A Magnussen
MGI ID:	MGI:5476527
Synonyms:	-60/IRBEA4-TA-CFP
Gene:	Gt(ROSA)26Sor  Location: Chr6:113044389-113054205 bp, - strand  Genetic Position: Chr6, 52.73 cM
Alliance:	Gt(ROSA)26Sortm5.1(Smad7-CFP)Mgn page

Germline Transmission:	Earliest citation of germline transmission: J:195112
Parent Cell Line:	Not Specified (ES Cell)
Strain of Origin:	129S6/SvEvTac

Allele Type:    Targeted Mutation:    Insertion   Mutation details: This allele was created by RMCE in Gt(ROSA)26Sortm1Mgn. The original selections cassettes (puromycin N-acetyltransferase and a truncated form of HSV1 thymidine kinase and EM7-driven neomycin resistance) were replaced by a cassette composed of ROSA26 upstream sequence deleted from the start codon to -60, a 4X multimer of the IBRE element from the Smad7 gene controlling expression of the CFP reporter. An upstream FLP flanked hygromycin selection cassette was subsequently removed by flp recombinase. (J:195112)

Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:	Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gt(ROSA)26Sor Mutation:	942 strains or lines available

Original:	J:195112 Serup P, et al., Partial promoter substitutions generating transcriptional sentinels of diverse signaling pathways in embryonic stem cells and mice. Dis Model Mech. 2012 Nov;5(6):956-66
All:	1 reference(s)