Gt(ROSA)26Sor^{tm2(OVA/EGFP)Dwir}
Targeted Allele Detail

Summary

• Symbol: Gt(ROSA)26Sor^{tm2(OVA/EGFP)Dwir}
• Name: gene trap ROSA 26, Philippe Soriano; targeted mutation 2, Dagmar Wirth
• MGI ID: MGI:5056498
• Synonyms: ROSAOVA
• Gene: Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
• Alliance: Gt(ROSA)26Sor^{tm2(OVA/EGFP)Dwir} page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:173806
• Parent Cell Line: IB10/E14IB10 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Conditional ready, Inserted expressed sequence, RMCE-ready, Reporter)
• Mutation: Insertion
• Mutation details: RMCE replaced the floxed luciferase gene in Gt(ROSA)26Sor^tm1Dwir with an inverted fusion protein of the hsp 73-capturing N-terminal viral J domain of SV40 T-Ag (cT77); 108-residue of the Ovalbumin fragment (aa 246-353 with a isoleucine to valine change at position 258) with well-characterized K^b- and A^d/b-binding epitopes (specifically recognized by OT-I or OT-II/D011 TCR), and the eGFP reporter. Cre-mediated recombination is required to achieve expression of the fusion protein. (J:173806)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Gt(ROSA)26Sor Mutation: 942 strains or lines available

References

• Original: J:173806 Sandhu U, et al., Strict control of transgene expression in a mouse model for sensitive biological applications based on RMCE compatible ES cells. Nucleic Acids Res. 2011 Jan 1;39(1):e1
• All: 4 reference(s)