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Targeted Allele Detail
Symbol: Col1a1tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae
Name: collagen, type I, alpha 1; targeted mutation 3, Rudolf Jaenisch
MGI ID: MGI:4430598
Synonyms: Col1a14F2A, Col1a1 4F2A, Col1a1-4F2A, Col1a1tetO-4F2A, Col1a1::tetO-4F2A
Gene: Col1a1  Location: Chr11:94827050-94843868 bp, + strand  Genetic Position: Chr11, 59.01 cM, cytoband D
Germline Transmission:  Earliest citation of germline transmission: J:157298
Parent Cell Line:  KH2 (ES Cell)
Strain of Origin:  (C57BL/6 x 129S4/SvJae)F1
Allele Type:    Targeted (Inducible, Inserted expressed sequence, RMCE-ready)
Mutation:    Insertion
Col1a1tm3(tetO-Pou5f1,-Sox2,-Klf4,-Myc)Jae expresses 4 genes
Mutation detailsRMCE on KH2 cells inserted a cassette that contains a loxP-flanked pgkNEOpolyA cassette, the tetracycline responsive element (TRE or tetO), the single polycistronic expression cassette 4F2A, and SV40 intron plus polyA signal into the 3'UTR. The 4F2A cassette contains the four mouse reprogramming genes Oct4 (Pou5f1; POU domain, class 5, transcription factor 1), Sox2 (SRY-box containing gene 2), Klf4 (Kruppel-like factor 4 (gut)), and c-Myc (Myc; myelocytomatosis oncogene) separated by three different viral 2A oligopeptides that mediate ribosomal skipping (P2A [from porcine teschovirus-1], T2A [from insect Thosea asigna virus], and E2A [equine rhinitis A virus], respectively). (J:157298)
View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Col1a1 Mutation:  141 strains or lines available
The allele was made in KH2 cells that carry Gt(ROSA)26Sortm1(rtTA*M2)Jae and Col1a1tm13(neo/hygro*)Jae (J:159351). This allele was generated with Col1a1tm13(neo/hygro*)Jae.
Original:  J:157298 Carey BW, et al., Single-gene transgenic mouse strains for reprogramming adult somatic cells. Nat Methods. 2010 Jan;7(1):56-9
All:  10 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.17
The Jackson Laboratory