Herc4^{Gt(RRH224)Byg}
Gene trapped Allele Detail

Summary

• Symbol: Herc4^{Gt(RRH224)Byg}
• Name: hect domain and RLD 4; gene trap RRH224, BayGenomics
• MGI ID: MGI:3768764
• Synonyms: Herc4^beta-geo
• Gene: Herc4 Location: Chr10:63079589-63153657 bp, + strand Genetic Position: Chr10, 32.56 cM, cytoband B4
• Alliance: Herc4^{Gt(RRH224)Byg} page

Mutation origin

• Mutant Cell Line: RRH224
• Germline Transmission: Earliest citation of germline transmission: J:128937
• Parent Cell Line: Other (see notes) (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Gene trapped (Null/knockout, Reporter)
• Mutation: Insertion of gene trap vector Vector: pGT0Lxf Vector Type: gene trap
• Mutation details: A BayGenomics gene trap vector (pGT0Lxf) was inserted into intron 13 creating an in frame fusion product with a beta-geo cassette. Gene inactivation was confirmed by Northern blots on testis RNA, and lacZ expression was confirmed in the testis of heterozygotes and homozygotes by beta-galactosidase staining. (J:128937)
• Sequence Tags: Sequence tag details (1 tag)

  Tag ID	GenBank ID	Method	Tag Location (trapped strand)*	Select
  RRH224	CC325672 ; (MGI Seq Detail)	5' RACE	Chr10:63118977-63123784 bp (+)
  * GRCm39; (strand of gene trap mutagenesis)

• Genome Context: Genome Browser view of this mutation;

  56 gene trap insertions have trapped this gene; View all gene trap sequence tags in this region

Expression

In Structures Affected by this Mutation:

1 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Herc4 Mutation: 64 strains or lines available

Notes

All BayGenomics gene trap mutations were generated in either CGR8 or E14TG2a (129P2/OlaHsd) parental ES cell lines, with the majority in subline E14TG2a.4. The specific ES cell line in which each mutation was made is not specified.

References

• Original: J:128937 Rodriguez CI, et al., Disruption of the ubiquitin ligase HERC4 causes defects in spermatozoon maturation and impaired fertility. Dev Biol. 2007 Dec 15;312(2):501-8
• All: 2 reference(s)