Chd4^tm1.1Kge
Targeted Allele Detail

Summary

• Symbol: Chd4^tm1.1Kge
• Name: chromodomain helicase DNA binding protein 4; targeted mutation 1.1, Katia Georgopoulos
• MGI ID: MGI:3641408
• Synonyms: Chd4^loxP, Mi-2beta^loxPF
• Gene: Chd4 Location: Chr6:125073144-125107554 bp, + strand Genetic Position: Chr6, 59.28 cM
• Alliance: Chd4^tm1.1Kge page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:109547
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A targeting vector was designed to insert loxP sites to flank exons 12 through 21 of the locus. Frt sites flanked a neo included in the vector in intron 11 that was subsequently deleted by FLP recombinase expression. The floxed locus encompasses the ATPase domain, which is required for the remodeling activity. Splicing of exon 11 to exon 22 would putatively generate an out of frame transcript, possibly resulting in a truncated protein. (J:109547)

Expression

In Mice Carrying this Mutation:	4 assay results 2 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	3 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Chd4 Mutation: 58 strains or lines available

References

• Original: J:109547 Williams CJ, et al., The chromatin remodeler Mi-2beta is required for CD4 expression and T cell development. Immunity. 2004 Jun;20(6):719-33
• All: 26 reference(s)