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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Chd4tm1.1Kge
targeted mutation 1.1, Katia Georgopoulos
MGI:3641408
Summary 4 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Chd4tm1.1Kge/Chd4tm1.2Kge
Tg(CAG-Bgeo/GFP)21Lbe/0
Tg(Lck-cre)1Cwi/0 		involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL MGI:3713384
cn2
 		Chd3tm1.1Cya/Chd3tm1.1Cya
Chd4tm1.1Kge/Chd4tm1.1Kge
Tg(Tek-cre)1Ywa/0 		involves: C57BL/6 * C57BL/6J * SJL MGI:6456933
cn3
 		Chd4tm1.1Kge/Chd4tm1.1Kge
Tg(Lck-cre)1Cwi/0 		involves: C57BL/6 * SJL MGI:3713383
cn4
 		Chd4tm1.1Kge/Chd4tm1.2Kge
Tg(Lck-cre)1Cwi/0 		involves: C57BL/6 * SJL MGI:3713382


Genotype
MGI:3713384
cn1
Allelic
Composition		 Chd4tm1.1Kge/Chd4tm1.2Kge
Tg(CAG-Bgeo/GFP)21Lbe/0
Tg(Lck-cre)1Cwi/0
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chd4tm1.1Kge mutation (0 available); any Chd4 mutation (58 available)
Chd4tm1.2Kge mutation (0 available); any Chd4 mutation (58 available)
Tg(CAG-Bgeo/GFP)21Lbe mutation (2 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cellular
abnormal cell cycle ( J:109547 )
• progressive decrease in cycling T cells is observed from second to third cell division in vitro, along with large number of nonblast T cells that appear to fail to enter cell cycle
decreased T cell proliferation ( J:109547 )
• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase

immune system
decreased T cell proliferation ( J:109547 )
• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase

hematopoietic system
decreased T cell proliferation ( J:109547 )
• a 5- to 7-fold decrease in proliferation is observed relative to wild-type cells in vitro after 48 hours of TCR/CD3 stimulation; fewer mutant cells are in S phase




Genotype
MGI:6456933
cn2
Allelic
Composition		 Chd3tm1.1Cya/Chd3tm1.1Cya
Chd4tm1.1Kge/Chd4tm1.1Kge
Tg(Tek-cre)1Ywa/0
Genetic
Background		 involves: C57BL/6 * C57BL/6J * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chd3tm1.1Cya mutation (0 available); any Chd3 mutation (101 available)
Chd4tm1.1Kge mutation (0 available); any Chd4 mutation (58 available)
Tg(Tek-cre)1Ywa mutation (6 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
cardiovascular system
abnormal blood vessel morphology ( J:294633 )
• vascular rupture in E11.5 embryos
• normal vasculature in E10.5 embryos
hemorrhage ( J:294633 )
• in E11.5 embryos

mortality/aging
embryonic lethality, complete penetrance ( J:294633 )
• most embryos die between age E10.5-11.5




Genotype
MGI:3713383
cn3
Allelic
Composition		 Chd4tm1.1Kge/Chd4tm1.1Kge
Tg(Lck-cre)1Cwi/0
Genetic
Background		 involves: C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chd4tm1.1Kge mutation (0 available); any Chd4 mutation (58 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased double-positive T cell number ( J:109547 )
• percentage of DP cells is reduced by 3-fold
arrested T cell differentiation ( J:109547 )
• differentiation of thymocytes stalls at the DN4 stage, but some do make the transition to DP eventually
• absolute number of double negative (DN) thymocytes is unaltered, or elevated despite decrease in thymus cellularity
increased CD8-positive, alpha-beta T cell number ( J:109547 )
• CD8 + T cells are increased to compensate for reduced DP cell numbers
spleen hypoplasia ( J:109547 )
• 2- to 5-fold reduction in T cells in spleen is observed; decreased numbers of CD8+ and CD4+ populations is noted
abnormal T cell physiology ( J:109547 )
• DP and CD4+ T cells express lower levels of CD4, and CD8+ cells range from CD4lo to CD4-
lymph node hypoplasia ( J:109547 )
• 2- to 5-fold reduction in T cells in lymph nodes is observed; decreased numbers of CD8+ and CD4+ populations is noted

hematopoietic system
decreased double-positive T cell number ( J:109547 )
• percentage of DP cells is reduced by 3-fold
arrested T cell differentiation ( J:109547 )
• differentiation of thymocytes stalls at the DN4 stage, but some do make the transition to DP eventually
• absolute number of double negative (DN) thymocytes is unaltered, or elevated despite decrease in thymus cellularity
increased CD8-positive, alpha-beta T cell number ( J:109547 )
• CD8 + T cells are increased to compensate for reduced DP cell numbers
spleen hypoplasia ( J:109547 )
• 2- to 5-fold reduction in T cells in spleen is observed; decreased numbers of CD8+ and CD4+ populations is noted
abnormal T cell physiology ( J:109547 )
• DP and CD4+ T cells express lower levels of CD4, and CD8+ cells range from CD4lo to CD4-




Genotype
MGI:3713382
cn4
Allelic
Composition		 Chd4tm1.1Kge/Chd4tm1.2Kge
Tg(Lck-cre)1Cwi/0
Genetic
Background		 involves: C57BL/6 * SJL
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Chd4tm1.1Kge mutation (0 available); any Chd4 mutation (58 available)
Chd4tm1.2Kge mutation (0 available); any Chd4 mutation (58 available)
Tg(Lck-cre)1Cwi mutation (3 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
thymus hypoplasia ( J:109547 )
• reduced in mutant mice

immune system
thymus hypoplasia ( J:109547 )
• reduced in mutant mice

endocrine/exocrine glands
thymus hypoplasia ( J:109547 )
• reduced in mutant mice




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Send questions and comments to User Support. 		last database update
04/30/2024
MGI 6.23 		The Jackson Laboratory