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Sox10tm2(rtTA)Weg
Targeted Allele Detail
Nomenclature
Symbol: Sox10tm2(rtTA)Weg
Name: SRY (sex determining region Y)-box 10; targeted mutation 2, Michael Wegner
MGI ID: MGI:3513104
Synonyms: Sox10rtTA
Gene: Sox10  Location: Chr15:79154913-79164490 bp, - strand  Genetic Position: Chr15, 37.7 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:94207
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout, Transactivator)
Mutation:    Insertion
 
Mutation detailsExons 3-5 (the entire coding region) were replaced with a reverse tetracycline controlled transactivator (rtTA) gene and a loxP-flanked Neo cassette, placing the start codon of the rtTA at the position of the endogenous start codon in exon 3, via homologous recombination. The Neo cassette was removed by crossing to mice expressing germline Cre-recombinase. Crossing to mice expressing lacZ under the control of the tetracycline inducible promoter and then treating with doxycycline resulted in X-gal staining (indicating rtTA activity) that recapitulated Sox10 expression. (J:94207)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 3 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Sox10 Mutation:  7 strains or lines available
References
Original:  J:94207 Ludwig A, et al., Sox10-rtTA mouse line for tetracycline-inducible expression of transgenes in neural crest cells and oligodendrocytes. Genesis. 2004 Nov;40(3):171-5
All:  10 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
11/12/2019
MGI 6.14
The Jackson Laboratory