Nodal^tm4Rob
Targeted Allele Detail

Summary

• Symbol: Nodal^tm4Rob
• Name: nodal growth differentiation factor; targeted mutation 4, Elizabeth J Robertson
• MGI ID: MGI:2668418
• Synonyms: Nodal^deltaPEE
• Gene: Nodal Location: Chr10:61253751-61261117 bp, + strand Genetic Position: Chr10, 32.21 cM
• Alliance: Nodal^tm4Rob page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:84300
• Parent Cell Line: CCE/EK.CCE (ES Cell)
• Strain of Origin: 129S/SvEv-Gpi1^c

Mutation description

• Allele Type: Targeted (Hypomorph, Modified regulatory region)
• Mutation: Other
• Mutation details: A hypomorphic allele was generated by the deletion of the proximal epiblast enhancer (PEE), a 2 kb element located about 10 kb upstream of the first exon. The PEE element was initially replaced with a floxed PGK-hygro cassette that was subsequently removed in vitro, leaving a single loxP in its place. (J:84300)

Expression

In Structures Affected by this Mutation:

5 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nodal Mutation: 41 strains or lines available

References

• Original: J:84300 Vincent SD, et al., Cell fate decisions within the mouse organizer are governed by graded Nodal signals. Genes Dev. 2003 Jul 1;17(13):1646-62
• All: 2 reference(s)