Phenotypes associated with this allele

• Allele Symbol: Nodal^tm4Rob
• Allele Name: targeted mutation 4, Elizabeth J Robertson
• Allele ID: MGI:2668418
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Nodal^tm4Rob/Nodal^tm4Rob	involves: 129/Sv * 129S/SvEv * C57BL/6	MGI:3689506
ht2	Nodal^TgR(MPSVNeo)413.dRob/Nodal^tm4Rob	involves: 129S/SvEv * C57BL/6	MGI:2668420
cx3	Eomes^tm1.1Rob/Eomes^+ Nodal^tm4Rob/Nodal^tm4Rob	involves: 129S/SvEv * C57BL/6 * CBA	MGI:3775739

Genotype
MGI:3689506

hm1

• Allelic Composition: Nodal^tm4Rob/Nodal^tm4Rob
• Genetic Background: involves: 129/Sv * 129S/SvEv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

perinatal lethality, complete penetrance ( J:84300 )

• embryos die late in gestation or at birth due to spectrum of complex defects

prenatal lethality, incomplete penetrance ( J:84300 )

• only 50% of homozygotes survive to term; remainder die late in gestation

cardiovascular system

abnormal heart development ( J:84300 )

• ~50% of homozygotes die late in gestation or at birth from spectrum of defects including heart abnormalities

craniofacial

abnormal craniofacial development ( J:84300 )

• ~50% of homozygotes die late in gestation or at birth from spectrum of defects including craniofacial defects

Genotype
MGI:2668420

ht2

• Allelic Composition: Nodal^{TgR(MPSVNeo)413.dRob}/Nodal^tm4Rob
• Genetic Background: involves: 129S/SvEv * C57BL/6

mortality/aging

embryonic lethality between somite formation and embryo turning, complete penetrance ( J:84300 )

• embryos develop severe anterior truncations at E8.5

embryo

rostral body truncation ( J:84300 )

• embryos develop severe anterior truncations at E8.5

abnormal embryonic neuroepithelium morphology ( J:84300 )

• at E9.5, anterior neurectoderm fails to proliferate and is filled with pyknotic nuclei

cardiovascular system

abnormal heart looping ( J:84300 )

• heart tube is abnormally looped

pericardial edema ( J:84300 )

• pericardium is often edematous

enlarged pericardium ( J:84300 )

• pericardium is often enlarged

digestive/alimentary system

abnormal foregut morphology ( J:84300 )

• gut tube forms but is poorly elaborated in anterior region; instead of extending to level of Rathke's pouch like wild-type, no gut tissue is evident at this level in mutants and is absent anterior to level of heart

homeostasis/metabolism

pericardial edema ( J:84300 )

• pericardium is often edematous

nervous system

abnormal embryonic neuroepithelium morphology ( J:84300 )

• at E9.5, anterior neurectoderm fails to proliferate and is filled with pyknotic nuclei

absent midbrain ( J:84300 )

• midbrain regions are not specified

absent forebrain ( J:84300 )

• forebrain regions are not specified

decreased embryonic neuroepithelial cell proliferation ( J:84300 )

• the anterior neurectoderm fails to proliferate

Genotype
MGI:3775739

cx3

• Allelic Composition: Eomes^tm1.1Rob/Eomes⁺; Nodal^tm4Rob/Nodal^tm4Rob
• Genetic Background: involves: 129S/SvEv * C57BL/6 * CBA

normal phenotype

no abnormal phenotype detected ( J:131055 )

• normal Mendelian numbers are recovered during embryogenesis