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Targeted Allele Detail
Symbol: Zeb1tm1Yhi
Name: zinc finger E-box binding homeobox 1; targeted mutation 1, Yujiro Higashi
MGI ID: MGI:1857515
Synonyms: deltaEF1deltaC727
Gene: Zeb1  Location: Chr18:5591860-5775467 bp, + strand  Genetic Position: Chr18, 4.42 cM
Abnormal thymus architecture in Zeb1tm1Yhi/Zeb1tm1Yhi mice

Show the 1 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:39865
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
Mutation detailsThe insertion of a neomycin selection cassette into exon 6 introduced a stop codon that precludes translation of the essential zinc finger DNA-binding domain. Northern blot analysis of total embryonic RNA showed the presence of longer transcripts, resulting from the insertion of neo. Western blot analysis using an anti-serum directed against the amino terminus of the encoded protein confirmed the presence of a truncated protein in extracts from homozygous mutant embryos. (J:39865)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 8 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Zeb1 Mutation:  43 strains or lines available
Original:  J:39865 Higashi Y, et al., Impairment of T cell development in deltaEF1 mutant mice. J Exp Med. 1997 Apr 21;185(8):1467-79
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.21
The Jackson Laboratory