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Gene Expression Data
Assay Details
Assay
Reference: J:83255 Chi CL, et al., The isthmic organizer signal FGF8 is required for cell survival in the prospective midbrain and cerebellum. Development. 2003 Jun;130(12):2633-44
Assay type: Immunohistochemistry
MGI Accession ID: MGI:2677311
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: anti-neurofilament clone 2H3
Detection system: Secondary antibody coupled to horseradish peroxidase
Results
Specimen 2G: embryonic day 10.5 (more )
Note: Authors state that heterozygous and wild-type littermates were used as control embryos.
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 2G
TS17: facial ganglion Present 2G
TS17: trigeminal ganglion Present 2G
TS17: central nervous system Present Regionally restricted 2G Expression in oculomotor nerve III, trochlear nerve IV, trigeminal nerve V, facial nerve VII and acoustic nerve VIII.

Specimen 2H: embryonic day 10.5; Fgf8tm1.1Mrt/Fgf8tm1.1Mrt (more )
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 2H
TS17: facial ganglion Present 2H
TS17: central nervous system Present Regionally restricted 2H Expression in oculomotor nerve III, facial nerve VII and acoustic nerve VIII. Trochlear nerve IV is absent.

Specimen 2I: embryonic day 10.5; Fgf8tm1.1Mrt/Fgf8tm1.4Mrt (more )
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 2I
TS17: facial ganglion Present 2I
TS17: trigeminal ganglion Present 2I
TS17: central nervous system Present Regionally restricted 2I Expression in facial nerve VII and acoustic nerve VIII. Oculomotor nerve III, trochlear nerve IV and the r1-derived component of trigeminal nerve V are absent or severely truncated.

Specimen 2J: embryonic day 10.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, En1tm2(cre)Wrst/En1+ (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS17: acoustic ganglion Present 2J
TS17: facial ganglion Present 2J
TS17: trigeminal ganglion Present 2J
TS17: central nervous system Present Regionally restricted 2J Expression in facial nerve VII and acoustic nerve VIII. Oculomotor nerve III, trochlear nerve IV and the r1-derived component of trigeminal nerve V are absent or severely truncated.


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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory