Gene Expression Data
Assay Details
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Assay
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Reference:
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J:79394
Ishibashi M, et al., A sonic hedgehog-dependent signaling relay regulates growth of diencephalic and mesencephalic primordia in the early mouse embryo. Development. 2002 Oct;129(20):4807-19
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:2676613
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Gene symbol:
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Ccnd1
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Gene name:
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cyclin D1
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Probe:
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Ccnd1 probe3
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
not shown:
embryonic day 9.0
(more  )
Note: 13 somite stage.
Specimen
not shown:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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13 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future hindbrain |
Present |
Regionally restricted |
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Broad expression in all three brain regions; highest levels in anterior midbrain, lower levels in diencephalon.
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| TS14: future midbrain |
Present |
Regionally restricted |
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Broad expression in all three brain regions; highest levels in anterior midbrain, lower levels in diencephalon.
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| TS14: prosencephalon |
Present |
Regionally restricted |
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Broad expression in all three brain regions; highest levels in anterior midbrain, lower levels in diencephalon.
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Specimen
3H:
embryonic day 9.0
(more )
Note: 17 somite stage.
Specimen
3H:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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17 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future hindbrain |
Present |
Regionally restricted |
3H
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Broad expression in all three brain regions; highest levels in anterior midbrain, lower levels in diencephalon.
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| TS14: future midbrain |
Present |
Regionally restricted |
3H
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Broad expression in all three brain regions; highest levels in anterior midbrain, lower levels in diencephalon.
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| TS14: prosencephalon |
Present |
Regionally restricted |
3H
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Broad expression in all three brain regions; highest levels in anterior midbrain, lower levels in diencephalon.
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Specimen
not shown:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Shhtm1Amc/Shhtm1Amc
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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13 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future diencephalon |
Absent |
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Expression undetectable throughout diencephalic region; anterior midbrain expression greatly reduced.
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| TS14: future midbrain |
Weak |
Regionally restricted |
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Expression undetectable throughout diencephalic region; anterior midbrain expression greatly reduced.
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Specimen
3I:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Shhtm1Amc/Shhtm1Amc
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Age:
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embryonic day 9.0
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Age Note:
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Embryonic age was assigned based on somite numbers reported in reference.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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17 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future diencephalon |
Absent |
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3I
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Expression undetectable throughout diencephalic region; anterior midbrain expression greatly reduced.
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| TS14: future midbrain |
Weak |
Regionally restricted |
3I
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Expression undetectable throughout diencephalic region; anterior midbrain expression greatly reduced.
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| TS14: tail bud |
Strong |
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3I
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Strong expression in tail maintained in mutants.
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Analysis Tools