Probe:	mTrps1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: head	Present	Regionally restricted	1A	Expression in facial region.
TS17: branchial arch	Present		1A
TS17: brain	Present		1A
TS17: forelimb bud	Present	Uniform	1A
TS17: hindlimb bud	Present	Uniform	1A

Structure	Level	Pattern	Image	Note
TS19: branchial arch	Present	Regionally restricted	1B	Expression in distal tips of the pharyngeal arches.
TS19: brain	Present		1B
TS19: forelimb bud	Present	Uniform	1B
TS19: hindlimb bud	Present	Uniform	1B
TS19: axial skeleton	Present	Regionally restricted	1B	Expression in segmental stripes.

Structure	Level	Pattern	Image	Note
TS20: head	Strong	Regionally restricted	1C	Expression in the distal region of the face.
TS20: mandibular process	Strong	Regionally restricted	1C	Expression in the distal region of the face.
TS20: maxillary process	Strong	Regionally restricted	1C	Expression in the distal region of the face.
TS20: vibrissa mesenchyme	Present		1C
TS20: brain	Present		1C
TS20: forelimb pre-cartilage condensation	Strong		1C
TS20: hindlimb pre-cartilage condensation	Strong		1C
TS20: axial skeleton	Present	Regionally restricted	1C	Expression in segmental stripes.

Structure	Level	Pattern	Image	Note
TS22: head	Strong	Regionally restricted	1D	Expression in the distal region of the face, the subcutaneous region of the snout, the area of maxilla and mandibula.
TS22: lower jaw	Strong	Regionally restricted	1D	Expression in the distal region of the face, the subcutaneous region of the snout, the area of maxilla and mandibula.
TS22: upper jaw	Strong	Regionally restricted	1D	Expression in the distal region of the face, the subcutaneous region of the snout, the area of maxilla and mandibula.
TS22: skin	Present	Regionally restricted	1D	Expression in skin surrounding the eye.
TS22: head mesenchyme	Present	Regionally restricted	1D	Expression in mesenchyme surrounding the whisker follicles.
TS22: integumental system	Present	Regionally restricted	1D	Expression in hair follicles.
TS22: brain	Present		1D
TS22: elbow joint primordium	Present		1D
TS22: knee joint primordium	Present		1D

Structure	Level	Pattern	Image	Note
TS20: skin	Present	Regionally restricted	2A	Expression in skin surrounding the eye.
TS20: head	Present	Regionally restricted	2A	Expression in connective tissue of the head.

Structure	Level	Pattern	Image	Note
TS20: skin	Present		2B
TS20: occipital myotome	Present		2B

Structure	Level	Pattern	Image	Note
TS20: vibrissa mesenchyme	Present		2C
TS20: epidermis	Absent		2C
TS20: nasal septum mesenchyme	Present	Regionally restricted	2C	Expression in the pre-cartilagenous condensation.
TS20: trachea	Present		2C

Structure	Level	Pattern	Image	Note
TS20: vibrissa mesenchyme	Present		2D
TS20: epidermis	Absent		2D
TS20: nasal septum mesenchyme	Present	Regionally restricted	2D	Expression in the precartilagenous condensation.

Structure	Level	Pattern	Image	Note
TS20: axial skeleton	Strong	Regionally restricted	2E	Expression in intervertebral disk.
TS20: elbow mesenchyme	Strong	Regionally restricted	2E	Expression in joint.
TS20: humerus pre-cartilage condensation	Weak		2E

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present	Regionally restricted	2F	Expression is lining the ribs and vertebrae.
TS20: vertebral cartilage condensation	Present	Regionally restricted	2F	Expression is lining the ribs and vertebrae.
TS20: axial skeleton	Strong	Regionally restricted	2F	Expression in intervertebral disk.

Structure	Level	Pattern	Image	Note
TS20: axial skeleton	Strong	Regionally restricted	2G	Expression in intervertebral disk.

Structure	Level	Pattern	Image	Note
TS20: rib pre-cartilage condensation	Present	Regionally restricted	2H	Expression is lining the ribs and vertebrae.
TS20: vertebral cartilage condensation	Present	Regionally restricted	2H	Expression is lining the ribs and vertebrae.
TS20: notochord	Absent		2H
TS20: axial skeleton	Strong	Regionally restricted	2H	Expression in intervertebral disk.

Structure	Level	Pattern	Image	Note
TS20: axial skeleton	Present		2I
TS20: shoulder mesenchyme	Strong	Regionally restricted	2I	Expression is in the joint.
TS20: humerus pre-cartilage condensation	Weak		2I

Structure	Level	Pattern	Image	Note
TS20: vibrissa mesenchyme	Present		2M
TS20: epidermis	Absent		2M

Structure	Level	Pattern	Image	Note
TS22: epidermis	Absent		2N
TS22: head mesenchyme	Present	Regionally restricted	2N	Expression in mesenchyme surrounding the whisker follicles.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Absent		2O
TS22: skin	Present	Regionally restricted	2O	Expression in hair follicles.
TS22: integumental system	Present	Regionally restricted	2O	Expression in hair follicles.

Structure	Level	Pattern	Image	Note
TS22: epidermis	Absent		3A
TS22: integumental system	Present	Regionally restricted	3A	Expression in hair follicles.
TS22: 3rd ventricle	Strong	Regionally restricted	3A	Expression in the inner layer.
TS22: midbrain	Present		3A
TS22: telencephalon	Present		3A
TS22: diaphragm	Present		3A
TS22: tongue muscle	Present		3A

Structure	Level	Pattern	Image	Note
TS22: skin	Present	Regionally restricted	3B	Expression in skin surrounding the eye.
TS22: integumental system	Present	Regionally restricted	3B	Expression in hair follicles.

Structure	Level	Pattern	Image	Note
TS22: skin	Present		3C
TS22: head mesenchyme	Present	Regionally restricted	3C	Expression in mesenchyme surrounding the whisker follicles.

Structure	Level	Pattern	Image	Note
TS22: tongue muscle	Present		3D
TS22: epidermis	Absent		3D

Structure	Level	Pattern	Image	Note
TS22: integumental system	Present	Regionally restricted	3E	Expression in hair follicles.
TS22: 3rd ventricle	Strong	Regionally restricted	3E	Expression in the inner layer.
TS22: midbrain	Present		3E
TS22: telencephalon	Present		3E

Structure	Level	Pattern	Image	Note
TS22: gut	Present	Regionally restricted	3F	Expression in mesenchyme, absent in endoderm.

Structure	Level	Pattern	Image	Note
TS22: lung mesenchyme	Present		3G
TS22: lung epithelium	Absent		3G

Structure	Level	Pattern	Image	Note
TS22: mesenchyme of metanephros	Present		3H
TS22: nephric duct of male, mesonephric portion	Present	Regionally restricted	3H	Expression in mesenchyme.
TS22: testis	Present	Regionally restricted	3H	Expression in interstitial cells.

Structure	Level	Pattern	Image	Note
TS22: cervical intervertebral disc	Strong		3I

Structure	Level	Pattern	Image	Note
TS22: heart	Absent
TS22: liver	Absent

Structure	Level	Pattern	Image	Note
TS24: forearm	Strong	Regionally restricted	4B	Expression in the periarticular chondrocytes. Expression in a stripe flanking the distal side of Ihh expression (shown in MGI:2663908), and in the ossification front.

Structure	Level	Pattern	Image	Note
TS24: forearm mesenchyme	Present	Regionally restricted	4E	Expression in the joint.
TS24: elbow joint	Present		4E
TS24: hand	Weak	Regionally restricted	4E	Expression in cartilage.
TS24: radius	Weak	Regionally restricted	4E	Expression in cartilage.
TS24: ulna	Weak	Regionally restricted	4E	Expression in cartilage.
TS24: forelimb phalanx	Present		4E	Expression overlaps with that of Gdf5 (shown in MGI:2663905).
TS24: forelimb digit mesenchyme	Present	Regionally restricted	4E	Expression in the joints separating the metacarpals. Expression overlaps with that of Gdf5 (shown in MGI:2663905).

Structure	Level	Pattern	Image	Note
TS22: forelimb phalanx cartilage condensation	Present			Expression overlaps with that of Gdf5 (shown in MGI:2663905).
TS22: hindlimb phalanx cartilage condensation	Present			Expression overlaps with that of Gdf5 (shown in MGI:2663905).
TS22: forelimb digit mesenchyme	Present	Regionally restricted		Expression in the joints separating the metacarpals and metatarsals. Expression overlaps with that of Gdf5 (shown in MGI:2663905).
TS22: hindlimb digit mesenchyme	Present	Regionally restricted		Expression in the joints separating the metacarpals and metatarsals. Expression overlaps with that of Gdf5 (shown in MGI:2663905).

Structure	Level	Pattern	Image	Note
TS24: hindlimb phalanx	Present			Expression overlaps with that of Gdf5 (shown in MGI:2663905).
TS24: hindlimb digit mesenchyme	Present	Regionally restricted		Expression in the joints separating the metatarsals. Expression overlaps with that of Gdf5 (shown in MGI:2663905).