Probe:	Sall1 probe2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS11: future brain	Present		1A	Expression is in a broad anterior region of the prospective head.

Structure	Level	Pattern	Image	Note
TS13: neural ectoderm	Present	Regionally restricted	1B	Expression is in a broad anterior region of the prospective head and in the neural groove.

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present	Regionally restricted	1C	Expression is at the midbrain-hindbrain boundary.
TS15: olfactory placode	Present		1C
TS15: future midbrain	Present	Regionally restricted	1C	Expression is in the anterior midbrain and at the midbrain-hindbrain boundary.
TS15: neural tube	Present	Regionally restricted	1C	Expression is in the epindymal and mantle layer.
TS15: tail neural tube	Present	Regionally restricted	1C	Expression is in the epindymal and mantle layer.
TS15: tail mesenchyme	Present		1C
TS15: branchial arch	Present		1C
TS15: mesonephros	Present		1C

Structure	Level	Pattern	Image	Note
TS15: future hindbrain	Present		1D
TS15: future midbrain	Present		1D
TS15: olfactory placode	Present		1D

Structure	Level	Pattern	Image	Note
TS15: oral region	Present	Regionally restricted	1E	Expression is in the mesenchyme surrounding the oral cavity.
TS15: 3rd branchial arch	Present		1E
TS15: branchial arch	Present	Regionally restricted	1E	The authors describe expression in the 4th arch.

Structure	Level	Pattern	Image	Note
TS15: neural tube	Present	Regionally restricted	1F	Expression is in the dorsal neural tube.
TS15: tail neural tube	Present	Regionally restricted	1F	Expression is in the dorsal neural tube.
TS15: 3rd branchial arch	Present		1F
TS15: mesonephros	Present		1F
TS15: branchial arch	Present	Regionally restricted	1F	The authors describe expression in the 4th arch.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present		1G
TS17: hindlimb bud	Present		1G
TS17: lens pit	Present		1G
TS17: medial-nasal process	Present		1G
TS17: telencephalon	Present		1G
TS17: hindbrain	Weak	Regionally restricted	1G	Expression at the midbrain-hindbrain boundary has decreased.
TS17: midbrain	Weak	Regionally restricted	1G	Expression at the midbrain-hindbrain boundary has decreased.
TS17: tail mesenchyme	Present		1G

Structure	Level	Pattern	Image	Note
TS17: lens pit	Present		1H

Structure	Level	Pattern	Image	Note
TS19: lens vesicle	Present		1I

Structure	Level	Pattern	Image	Note
TS19: telencephalon	Present	Regionally restricted	1J	Expression is in the olfactory bulbs.

Structure	Level	Pattern	Image	Note
TS19: genital tubercle	Strong		1K

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present	Regionally restricted	1L	Transcripts are prominent in the region of the telencephalon and the olfactory bulbs.

Structure	Level	Pattern	Image	Note
TS20: spinal cord mantle layer	Present		1M
TS20: spinal cord ventricular layer	Present		1M

Structure	Level	Pattern	Image	Note
TS20: diencephalon ventricular layer	Present	Regionally restricted	1N	Expression is in the tissue surrounding the ventricles.
TS20: hindbrain lateral wall	Present	Regionally restricted	1N	Expression is in the tissue surrounding the ventricles.
TS20: telencephalon ventricular layer	Present	Regionally restricted	1N	Expression is in the tissue surrounding the ventricles.
TS20: genital tubercle	Present	Regionally restricted	1N	Expression is in the epithelium and mesenchyme.
TS20: esophagus mesenchyme	Present		1N , 1O
TS20: trachea mesenchyme	Present		1N , 1O
TS20: mesenchyme of metanephros	Present	Regionally restricted	1P , 1N	Expression is in the medullar region.
TS20: metanephros	Present	Regionally restricted	1P , 1N	Expression is in the cortical region.

Structure	Level	Pattern	Image	Note
TS26: metanephros	Present		1Q
TS26: glomerular tuft	Present		1R , 1Q
TS26: renal cortex	Present	Regionally restricted	1R , 1Q	Expression is in the cortex cells subadjacent to the fibrous capsule.
TS26: cortical renal tubule	Present		1Q , 1S

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Graded	2A	Expression is more prominent in the posterior region.
TS17: hindlimb bud	Present	Graded	2A	Expression is more prominent in the posterior region.

Structure	Level	Pattern	Image	Note
TS19: footplate apical ectodermal ridge	Present	Uniform	2B
TS19: handplate apical ectodermal ridge	Present	Uniform	2B
TS19: footplate mesenchyme	Present	Regionally restricted	2B	Expression is divided into a proximal domain and one underlying the AER.
TS19: handplate mesenchyme	Present	Regionally restricted	2B	Expression is divided into a proximal domain and one underlying the AER.

Structure	Level	Pattern	Image	Note
TS19: handplate apical ectodermal ridge	Present	Uniform	2C
TS19: handplate mesenchyme	Present	Regionally restricted	2C	Expression is in the mesenchyme underlying the AER and in the dorsal and ventral mesenchyme.

Structure	Level	Pattern	Image	Note
TS19: footplate mesenchyme	Present	Regionally restricted	2D	Expression is anterior and posterior.

Structure	Level	Pattern	Image	Note
TS20: foot mesenchyme	Present	Regionally restricted	2E	The distance between the proximal and distal expression domain has increased from 11.5dpc.
TS20: hand mesenchyme	Present	Regionally restricted	2E	The distance between the proximal and distal expression domain has increased from 11.5dpc.
TS20: forelimb interdigital region	Present		2E
TS20: hindlimb interdigital region	Present		2E

Structure	Level	Pattern	Image	Note
TS20: interdigital region between forelimb digits 2 and 3 mesenchyme	Present		2F
TS20: interdigital region between forelimb digits 3 and 4 mesenchyme	Present		2F
TS20: hand epithelium	Present	Regionally restricted	2F	Expression persists in the AER.

Structure	Level	Pattern	Image	Note
TS20: foot mesenchyme	Present	Regionally restricted	2G	Expression is dorsal and ventral.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit mesenchyme	Present	Regionally restricted	2H upper	Expression is in the mesenchyme underlying the phalangeal tips.
TS21: forelimb	Weak	Regionally restricted	2H upper	Weak expression in proximal part of limb bud.

Structure	Level	Pattern	Image	Note
TS21: hindlimb digit mesenchyme	Present	Regionally restricted	2H lower	Expression is in the mesenchyme uderlying the phalangeal tips.
TS21: hindlimb	Weak	Regionally restricted	2H lower	Weak expression in proximal part of limb bud.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit 2 mesenchyme	Present	Regionally restricted	2I	Expression is ventral.
TS21: forelimb digit 3 mesenchyme	Present	Regionally restricted	2I	Expression is ventral.

Structure	Level	Pattern	Image	Note
TS21: forelimb digit 3 mesenchyme	Present	Regionally restricted	2J	Expression is in the ventral mesenchyme underlying the phylangeal tip.

Structure	Level	Pattern	Image	Note
TS22: forelimb digit mesenchyme	Weak	Regionally restricted	2K upper	Weak expression in distal digital tip.

Structure	Level	Pattern	Image	Note
TS22: hindlimb digit mesenchyme	Weak	Regionally restricted	2K lower	Weak expression in distal digit tips.

Structure	Level	Pattern	Image	Note
TS22: hand mesenchyme	Present	Regionally restricted	2L	Expression is in the AER and the underlying mesenchyme.
TS22: hand skin	Present	Regionally restricted	2L	Expression is in the AER and the underlying mesenchyme.

Structure	Level	Pattern	Image	Note
TS22: foot	Present	Regionally restricted	2M	Expression is on the ventral side of the proximal region.