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Gene Expression Data
Assay Details
Assay
Reference: J:41878 Zoltewicz JS, et al., oto is a homeotic locus with a role in anteroposterior development that is partially redundant with Lim1. Development. 1999 Nov;126(22):5085-95
Assay type: RNA in situ
MGI Accession ID: MGI:1346416
Gene symbol: Nkx2-1
Gene name: NK2 homeobox 1
Probe: Nkx2.1 probe7
Probe preparation: RNA
Visualized with: Alkaline phosphatase
Results
Specimen 3E: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 3E Expression is present in the ventral region of the anterior diencephalon.
TS15: telencephalon Present Regionally restricted 3E Expression is present in the ventral telencephalon.

Specimen 3F: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 3F Expression is present in the ventral region of the anterior diencephalon.
TS15: telencephalon Present Regionally restricted 3F Expression is present in the ventral telencephalon.

Specimen 3G: embryonic day 9.5; Pgap1oto/Pgap1+ (more )
Note: moderately affected oto mutant
Structure Level Pattern Image Note
TS15: telencephalon Absent 3G Expression is reduced or lost in oto mutants, indicating a loss of ventral telencephalon.
TS15: diencephalon Present Regionally restricted 3G Expression in this mutant is the same as in wild-type embryos.

Specimen 3H: embryonic day 9.5; Pgap1oto/Pgap1+ (more )
Note: moderately affected oto mutant
Structure Level Pattern Image Note
TS15: telencephalon Absent 3H Expression is reduced or lost in oto mutants, indicating a loss of ventral telencephalon.
TS15: diencephalon Present Regionally restricted 3H Expression in this mutant is the same as in wild-type embryos.

Specimen 3I: embryonic day 9.5; Pgap1oto/Pgap1+ (more )
Note: severely affected oto mutant
Structure Level Pattern Image Note
TS15: telencephalon Absent 3I Expression is reduced or lost in oto mutants, indicating a loss of ventral telencephalon.
TS15: diencephalon Present Regionally restricted 3I The persistance of expression indicates that most of the anterior diencephalon is present. However, the anterior midline of the diencephalon, the preoptic area and the pituitary region are apparently reduced or absent.

Specimen 3J: embryonic day 9.5; Pgap1oto/Pgap1+ (more )
Note: severely affected oto mutant
Structure Level Pattern Image Note
TS15: telencephalon Absent 3J Expression is reduced or lost in oto mutants, indicating a loss of ventral telencephalon.
TS15: diencephalon Present Regionally restricted 3J The persistance of expression indicates that most of the anterior diencephalon is present. However, the anterior midline of the diencephalon, the preoptic area and the pituitary region are apparently reduced or absent.

Specimen 6C: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 6C Expression is present in the ventral region of the anterior diencephalon.
TS15: telencephalon Present Regionally restricted 6C Expression is present in the ventral telencephalon.

Specimen 6D: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 6D Expression is present in the ventral region of the anterior diencephalon.
TS15: telencephalon Present Regionally restricted 6D Expression is present in the ventral telencephalon.

Specimen 6E: embryonic day 9.5; Lhx1tm1Bhr/Lhx1+, Pgap1oto/Pgap1+ (more )
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 6E Expression in this mutant is the same as in wild-type embryos.
TS15: telencephalon Present Regionally restricted 6E There is an anterior reduction of staining in the telencephalon, indicating a loss of in telencephalon.

Specimen 6F: embryonic day 9.5; Lhx1tm1Bhr/Lhx1+, Pgap1oto/Pgap1+ (more )
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 6F Expression in this mutant is the same as in wild-type embryos.
TS15: telencephalon Present Regionally restricted 6F There is an anterior reduction of staining in the telencephalon, indicating a loss of in telencephalon.

Specimen not shown: embryonic day 9.5; Lhx1tm1Bhr/Lhx1+, Pgap1oto/Pgap1oto (more )
Note: severely affected oto homozygote, Lim1 heterozygote
Structure Level Pattern Image Note
TS15: diencephalon Absent
TS15: telencephalon Absent

Specimen 6G: embryonic day 9.5; Lhx1tm1Bhr/Lhx1+, Pgap1oto/Pgap1oto (more )
Note: slightly less affected oto homozygote, Lim1 heterozygote
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 6G Expression is reduced in the ventral diencephalon compared to oto homozygotes.

Specimen 6H: embryonic day 9.5; Lhx1tm1Bhr/Lhx1+, Pgap1oto/Pgap1oto (more )
Note: slightly less affected oto homozygote, Lim1 heterozygote
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 6H Expression is reduced in the ventral diencephalon compared to oto homozygotes.

Specimen 6I: embryonic day 9.5; Lhx1tm1Bhr/Lhx1+, Pgap1oto/Pgap1oto (more )
Note: slightly less affected oto homozygote, Lim1 heterozygote
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 6I A small portion of the rostral diencephalon is retained as a residual expression domain.

Specimen 6J: embryonic day 9.5; Lhx1tm1Bhr/Lhx1+, Pgap1oto/Pgap1oto (more )
Note: slightly less affected oto homozygote, Lim1 heterozygote
Structure Level Pattern Image Note
TS15: diencephalon Present Regionally restricted 6J A small portion of the rostral diencephalon is retained as a residual expression domain.


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last database update
05/14/2024
MGI 6.23
The Jackson Laboratory