Probe:	KL-M1
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS17: head mesenchyme	Present	Regionally restricted	5A	Expression was detected in the mesenchyme between the telencephalon and the olfactory pit, and in the mesenchyme surrounding the otic vesicle.
TS17: trunk mesenchyme	Present	Regionally restricted	5A	Dermatomes express in their dorsal portions. Expression was evident in dermatomes from the first somite to the midtrunk level.

Structure	Level	Pattern	Image	Note
TS17: 1st branchial arch	Present	Regionally restricted	5B	Expression in the first and second branchial arch was confined to the most posterior proximal edges.
TS17: 2nd branchial arch	Present	Regionally restricted	5B	Expression in the first and second branchial arch was confined to the most posterior proximal edges.
TS17: trunk mesenchyme	Present	Regionally restricted	5B	Expression was detected in dermatomes from the first somites back to somites at the hindlimb level.

Structure	Level	Pattern	Image	Note
TS18: telencephalon	Present		5C
TS18: head mesenchyme	Absent		5C
TS18: trunk paraxial mesenchyme	Present	Regionally restricted	5C	Expression was diminished in the cervical somites, whereas the dorsal dermatome of all the posterior somites down to the base to the tail were strongly positive.

Structure	Level	Pattern	Image	Note
TS18: trunk mesenchyme	Present	Regionally restricted	5D	No positive cells were seen in the migrating staging area.

Structure	Level	Pattern	Image	Note
TS19: head mesenchyme	Absent		5E
TS19: trunk somite	Absent		5E
TS19: tail somite	Present		5E
TS19: telencephalon	Present		5E

Structure	Level	Pattern	Image	Note
TS19: hindlimb bud	Present	Regionally restricted	5F	Expression was present in the posterior region of the hindlimb bud.

Structure	Level	Pattern	Image	Note
TS17: trunk mesenchyme	Present	Regionally restricted	5G	In progressively older dermatomal tissue, the location of expression was shifted from the mediodorsal portion to a more dorsal location in the epithelial dermatome.

Structure	Level	Pattern	Image	Note
TS17: trunk mesenchyme	Present	Regionally restricted	5I	In progressively older dermatomal tissue, the location of expression was shifted from the mediodorsal portion to a more dorsal location in the epithelial dermatome.

Structure	Level	Pattern	Image	Note
TS15: branchial arch	Present
TS15: head mesenchyme	Present
TS15: mesonephros	Present
TS15: tail	Present
TS15: 3rd branchial arch mesenchyme	Present	Regionally restricted		Expression was detected in the mesenchyme posterior to the last branchial arch.
TS15: gut	Present	Regionally restricted		Expression was detected in part of the gut.
TS15: trunk paraxial mesenchyme	Absent			No expression was detected within the somitic tissue of the trunk.

Structure	Level	Pattern	Image	Note
TS20: telencephalon	Present
TS20: foot	Present	Regionally restricted		New expression was detected in the interdigital mesenchyme of the limb buds.
TS20: hand	Present	Regionally restricted		New expression was detected in the interdigital mesenchyme of the limb buds.
TS20: tail somite	Present	Regionally restricted		Expression was detected in the dorsal dermatome only in the tail somites.