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Gene Expression Data
Assay Details
Assay
Reference: J:37336 Chapman DL, et al., Tbx6, a mouse T-Box gene implicated in paraxial mesoderm formation at gastrulation. Dev Biol. 1996 Dec 15;180(2):534-42
Assay type: RNA in situ
MGI Accession ID: MGI:1340265
Gene symbol: Tbx6
Gene name: T-box 6
Probe: w32
Probe preparation: Antisense labelled with digoxigenin RNA
Visualized with: Alkaline phosphatase
Results
Specimen 2B,C: embryonic day 7.5 (more )
Structure Level Pattern Image Note
TS11: embryo mesoderm Present Regionally restricted 2C , 2B Expression is in the primitive streak from the node to the base of the allantois. Expression extends laterally in the newly recruited paraxial mesoderm.
TS11: primitive streak Present Regionally restricted 2C , 2B Expression is in the primitive streak from the node to the base of the allantois. Expression extends laterally in the newly recruited paraxial mesoderm.

Specimen 2E,F: embryonic day 8.5 (more )
Structure Level Pattern Image Note
TS12: trunk unsegmented mesenchyme Present Regionally restricted 2F , 2E Expression is in the unsegmented, presomitic mesoderm of the tail surrounding the caudal neural plate.

Specimen 2H: embryonic day 9.5 (more )
Structure Level Pattern Image Note
TS15: tail unsegmented mesenchyme Present Regionally restricted 2H Expression is in the caudal region extending from the area of the open neural tube rostrally to the level of neural tube closure.
TS15: hindgut mesenchyme Present 2H

Specimen 2K: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: tail Present 2K

Specimen 2L: embryonic day 11.5 (more )
Structure Level Pattern Image Note
TS19: tail Present 2L

Specimen 2M: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: tail Present 2M

Specimen 2N: embryonic day 13.5 (more )
Structure Level Pattern Image Note
TS21: tail Absent 2N

Specimen 3A: embryonic day 7.5; T/T+, th2/t+ (more )
Note: This embryo is a compound heterozygote T/th2. The th2 haplotype was used as a marker chromosome. This genotype prevents recombination between T and Tcp1 and allows the use of a Tcp1 polymorphism to assign genotype.
Structure Level Pattern Image Note
TS11: embryo mesoderm Present Regionally restricted 3A Expression extends caudally from the node to the base of the allantois and in the paraxial mesoderm surrounding the streak.
TS11: primitive streak Present Regionally restricted 3A Expression extends caudally from the node to the base of the allantois and in the paraxial mesoderm surrounding the streak.

Specimen 3B: embryonic day 7.5; T/T (more )
Structure Level Pattern Image Note
TS11: embryo mesoderm Present Regionally restricted 3B Expression extends caudally from the node to the base of the allantois and in the paraxial mesoderm surrounding the streak.
TS11: primitive streak Present Regionally restricted 3B Expression extends caudally from the node to the base of the allantois and in the paraxial mesoderm surrounding the streak.

Specimen 3C,D: embryonic day 8.5; T/T (more )
Structure Level Pattern Image Note
TS13: embryo Absent 3D , 3C Abnormal (top) embryos show no expression.

Specimen 3E: embryonic day 9.5; T/T (more )
Structure Level Pattern Image Note
TS15: embryo Absent 3E Abnormal embryos (left) show no expression.

Specimen 3F: embryonic day 11.5; T/T+, th2/t+ (more )
Note: This embryo is a compound heterozygote T/th2. The th2 haplotype was used as a marker chromosome. This genotype prevents recombination between T and Tcp1 and allows the use of a Tcp1 polymorphism to assign genotype.
Structure Level Pattern Image Note
TS19: tail Present 3F Despite morphological defects, expression is normal.

Specimen Not Shown: embryonic day 6.5 (more )
Structure Level Pattern Image Note
TS9: embryo Absent

Specimen Not Shown: embryonic day 7.0 (more )
Structure Level Pattern Image Note
TS10: primitive streak Present


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
10/22/2024
MGI 6.24
The Jackson Laboratory