Gene Expression Data
Assay Details
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Assay
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Reference:
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J:37336
Chapman DL, et al., Tbx6, a mouse T-Box gene implicated in paraxial mesoderm formation at gastrulation. Dev Biol. 1996 Dec 15;180(2):534-42
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:1340265
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Gene symbol:
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Tbx6
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Gene name:
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T-box 6
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Probe:
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w32
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
2B,C:
embryonic day 7.5
(more  )
Specimen
2B,C:
(close  )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo mesoderm |
Present |
Regionally restricted |
2C
,
2B
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Expression is in the primitive streak from the node to the base of the allantois. Expression extends laterally in the newly recruited paraxial mesoderm.
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| TS11: primitive streak |
Present |
Regionally restricted |
2C
,
2B
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Expression is in the primitive streak from the node to the base of the allantois. Expression extends laterally in the newly recruited paraxial mesoderm.
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Specimen
2E,F:
embryonic day 8.5
(more )
Specimen
2E,F:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 8.5
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Age Note:
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Theiler stage of this specimen assigned by curators from EMAGE.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: trunk unsegmented mesenchyme |
Present |
Regionally restricted |
2F
,
2E
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Expression is in the unsegmented, presomitic mesoderm of the tail surrounding the caudal neural plate.
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Specimen
2H:
embryonic day 9.5
(more )
Specimen
2H:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2K:
embryonic day 10.5
(more )
Specimen
2K:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2L:
embryonic day 11.5
(more )
Specimen
2L:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2M:
embryonic day 12.5
(more )
Specimen
2M:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
2N:
embryonic day 13.5
(more )
Specimen
2N:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
3A:
embryonic day 7.5; T/T +, th2/t +
(more )
Note: This embryo is a compound heterozygote T/th2. The th2 haplotype was used as a marker chromosome. This genotype prevents recombination between T and Tcp1 and allows the use of a Tcp1 polymorphism to assign genotype.
Specimen
3A:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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T/T+
th2/t+
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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This embryo is a compound heterozygote T/th2. The th2 haplotype was used as a marker chromosome. This genotype prevents recombination between T and Tcp1 and allows the use of a Tcp1 polymorphism to assign genotype.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo mesoderm |
Present |
Regionally restricted |
3A
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Expression extends caudally from the node to the base of the allantois and in the paraxial mesoderm surrounding the streak.
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| TS11: primitive streak |
Present |
Regionally restricted |
3A
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Expression extends caudally from the node to the base of the allantois and in the paraxial mesoderm surrounding the streak.
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Specimen
3B:
embryonic day 7.5; T/ T
(more )
Specimen
3B:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 7.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: embryo mesoderm |
Present |
Regionally restricted |
3B
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Expression extends caudally from the node to the base of the allantois and in the paraxial mesoderm surrounding the streak.
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| TS11: primitive streak |
Present |
Regionally restricted |
3B
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Expression extends caudally from the node to the base of the allantois and in the paraxial mesoderm surrounding the streak.
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Specimen
3C,D:
embryonic day 8.5; T/ T
(more )
Specimen
3C,D:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 8.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: embryo |
Absent |
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3D
,
3C
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Abnormal (top) embryos show no expression.
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Specimen
3E:
embryonic day 9.5; T/ T
(more )
Specimen
3E:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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T/T
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Age:
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embryonic day 9.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: embryo |
Absent |
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3E
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Abnormal embryos (left) show no expression.
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Specimen
3F:
embryonic day 11.5; T/T +, th2/t +
(more )
Note: This embryo is a compound heterozygote T/th2. The th2 haplotype was used as a marker chromosome. This genotype prevents recombination between T and Tcp1 and allows the use of a Tcp1 polymorphism to assign genotype.
Specimen
3F:
(close )
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Genetic Background:
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Not Specified
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Mutant Allele(s):
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T/T+
th2/t+
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
|
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Note:
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This embryo is a compound heterozygote T/th2. The th2 haplotype was used as a marker chromosome. This genotype prevents recombination between T and Tcp1 and allows the use of a Tcp1 polymorphism to assign genotype.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: tail |
Present |
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3F
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Despite morphological defects, expression is normal.
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Specimen
Not Shown:
embryonic day 6.5
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 6.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Specimen
Not Shown:
embryonic day 7.0
(more )
Specimen
Not Shown:
(close )
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Genetic Background:
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Swiss Webster
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Age:
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embryonic day 7.0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Analysis Tools