Probe:	Pax3 probe10
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS15: trunk dermomyotome	Present	Regionally restricted	1a	Expression is seen in muscle progenitor cells that have left somites 9-12 at the level of the forelimb bud.

Structure	Level	Pattern	Image	Note
TS15: forelimb bud	Present	Regionally restricted	1b	Positive cells are dermomyotomal cells that are located in the proximal part of the forelimb, adjacent to somites 9-14.

Structure	Level	Pattern	Image	Note
TS16: hindlimb bud	Present	Regionally restricted	1c	Expressing cells have begun to delaminate from somites 26-29.

Structure	Level	Pattern	Image	Note
TS17: hindlimb bud	Present	Regionally restricted	1d	Expressing cells have begun to delaminate from adjacent somites to locate within the proximal part of the hindlimb bud.

Structure	Level	Pattern	Image	Note
TS17: forelimb bud	Present	Regionally restricted	1e	Expressing cells have migrated from adjacent somites into forelimb bud.

Structure	Level	Pattern	Image	Note
TS18: hindlimb bud	Present	Regionally restricted	1f	Expressing cells comprise hindlimb premuscle mass. A few positive cells still appear to be delaminating from somites 27,31 and 32.

Structure	Level	Pattern	Image	Note
TS16: trunk dermomyotome	Present	Regionally restricted	2d inset	Expression is in the lateral region of the dermomyotome.