Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:39351
de la Pompa JL, et al., Conservation of the Notch signalling pathway in mammalian neurogenesis. Development. 1997 Mar;124(6):1139-48
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:1269977
|
|
Gene symbol:
|
Ascl1
|
|
Gene name:
|
achaete-scute family bHLH transcription factor 1
|
|
|
Results
|
Specimen
5A:
embryonic day 8.5
(more  )
Specimen
5A:
(close  )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Present |
Spotted |
5A
|
Expression is in scattered cells of the midbrain region from which the first neurons of the CNS emerge.
|
| TS13: neural tube |
Present |
Regionally restricted |
5A
|
Expression present in the anterior portion of the neural tube.
|
Specimen
5B:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Rbpjtm1Kyo/Rbpjtm1Kyo
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5C:
embryonic day 8.5
(more )
Specimen
5C:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS13: future midbrain |
Present |
Spotted |
5C
|
Expression is in scattered cells of the midbrain region from which the first neurons of the CNS emerge.
|
| TS13: neural tube |
Present |
Regionally restricted |
5C
|
Expression present in the anterior portion of the neural tube.
|
Specimen
5D:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Rbpjtm1Kyo/Rbpjtm1Kyo
|
|
Age:
|
embryonic day 8.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5E:
embryonic day 9.0
(more )
Specimen
5E:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future midbrain |
Present |
Regionally restricted |
5E
|
Expression is restricted to the dorsal midbrain and to two patches at either side of the otic vesicle.
|
Specimen
5F/J:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Rbpjtm1Kyo/Rbpjtm1Kyo
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5G/I:
embryonic day 9.0
(more )
Specimen
5G/I:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future midbrain |
Present |
Regionally restricted |
5I
,
5G
|
Expression is restricted to the dorsal midbrain and to two patches at either side of the otic vesicle.
|
Specimen
5H/K:
(close )
|
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * CD-1)
|
|
Mutant Allele(s):
|
Notch1tm1Con/Notch1tm1Con
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
Specimen
5L:
embryonic day 9.0
(more )
Specimen
5L:
(close )
|
Genetic Background:
|
Not Specified
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section from whole mount
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future midbrain |
Present |
Regionally restricted |
5L
|
Expression restricted to the subventricular zone of the dorsal midbrain.
|
Specimen
5M:
(close )
|
Genetic Background:
|
involves: 129S2/SvPas * C57BL/6
|
|
Mutant Allele(s):
|
Rbpjtm1Kyo/Rbpjtm1Kyo
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future hindbrain |
Strong |
Regionally restricted |
5M
|
Expression is enhanced and its domain expanded dorsoventrally to the ventricular zone in the midbrain and hindbrain region.
|
| TS14: future midbrain |
Strong |
Regionally restricted |
5M
|
Expression is enhanced and its domain expanded dorsoventrally to the ventricular zone in the midbrain and hindbrain region.
|
Specimen
5N:
(close )
|
Genetic Background:
|
either: (involves: 129S1/Sv * 129X1/SvJ) or (involves: 129S1/Sv * 129X1/SvJ * C57BL/6) or (involves: 129S1/Sv * 129X1/SvJ * CD-1)
|
|
Mutant Allele(s):
|
Notch1tm1Con/Notch1tm1Con
|
|
Age:
|
embryonic day 9.0
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS14: future hindbrain |
Strong |
Regionally restricted |
5N
|
Expression is enhanced and its domain expanded dorsoventrally to the ventricular zone in the midbrain and hindbrain region.
|
| TS14: future midbrain |
Strong |
Regionally restricted |
5N
|
Expression is enhanced and its domain expanded dorsoventrally to the ventricular zone in the midbrain and hindbrain region.
|
|
Analysis Tools