About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:359864 Chen Y, et al., The Ednrb-Aim2-AKT axis regulates neural crest-derived melanoblast proliferation during early development. Development. 2024 Nov 15;151(22):dev202444
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7861728
Gene symbol: Pmel
Gene name: premelanosome protein
Antibody: anti-gp100 [HMB45] (ab787)
Detection system: Secondary antibody
Results
Specimen 2A Ednrb-/+ upper PMEL17: embryonic day 11.5; Ednrbtm1Myks/Ednrb+ (more )
Note: Double labeled: green - Pmel; red - Casp3 (assay MGI:7861730).
Structure Cell Type Level Pattern Image Note
TS19: trunk mesenchyme derived from neural crest melanoblast Present 2A -/+ upper PMEL17 , 2A -/+ upper merged , 2A Ednrb-/+ upper
Specimen 2A Ednrb-/+ lower PMEL17: embryonic day 11.5; Ednrbtm1Myks/Ednrb+ (more )
Note: Double labeled: green - Pmel; red - Mki67 (assay MGI:7861731).
Structure Cell Type Level Pattern Image Note
TS19: trunk mesenchyme derived from neural crest melanoblast Present 2A Ednrb-/+ lower , 2A -/+ lower merged , 2A -/+ lower PMEL17 Expression in melantoblasts colocalized with Mki67.
Specimen 2A Ednrb-/- upper PMEL17: embryonic day 11.5; Ednrbtm1Myks/Ednrbtm1Myks (more )
Note: Double labeled: green - Pmel; red - Casp3 (assay MGI:7861730).
Structure Cell Type Level Pattern Image Note
TS19: trunk mesenchyme derived from neural crest melanoblast Present 2A Ednrb-/- upper , 2A -/- upper merged , 2A -/- upper PMEL17
Specimen 2A Ednrb-/- lower PMEL17: embryonic day 11.5; Ednrbtm1Myks/Ednrbtm1Myks (more )
Note: Double labeled: green - Pmel; red - Mki67 (assay MGI:7861731).
Structure Cell Type Level Pattern Image Note
TS19: trunk mesenchyme derived from neural crest melanoblast Present 2A -/- lower PMEL17 , 2A -/- lower merged , 2A Ednrb-/- lower
Specimen 3E PMEL17: embryonic day 11.5; Aim2em2.1Gpt/Aim2em2.1Gpt, Ednrbtm1Myks/Ednrbtm1Myks (more )
Note: Double labeled: red - Pmel; green - Aim2 reporter (assay MGI:7861733).
Structure Cell Type Level Pattern Image Note
TS19: dorsal root ganglion melanoblast Present 3E lower Merge , 3E upper Merge , 3E upper PMEL17 , 3E lower PMEL17 Expression in melanoblasts co-localized with Aim3 reporter.
TS19: neural tube melanoblast Present 3E lower Merge , 3E upper Merge , 3E upper PMEL17 , 3E lower PMEL17 Expression in melanoblasts co-localized with Aim3 reporter.
Specimen 6C WT PMEL17: embryonic day 11.5 (more )
Note: Double labeled: green - Pmel; red - Mki67 (assay MGI:7861731).
Structure Cell Type Level Pattern Image Note
TS19: mesenchyme derived from neural crest melanoblast Present 6C WT PMEL17 , 6C WT merge , 6C WT
Specimen 6C Ednrb-/- PMEL17: embryonic day 11.5; Ednrbtm1Myks/Ednrbtm1Myks (more )
Note: Double labeled: green - Pmel; red - Mki67 (assay MGI:7861731).
Structure Cell Type Level Pattern Image Note
TS19: mesenchyme derived from neural crest melanoblast Present 6C Ednrb-/- merge , 6C Ednrb-/- , 6C Ednrb-/- PMEL17
Specimen 6C Ednrb-/-; Aim2-/- PMEL17: embryonic day 11.5; Aim2em2.1Gpt/Aim2em2.1Gpt, Ednrbtm1Myks/Ednrbtm1Myks (more )
Note: Double labeled: green - Pmel; red - Mki67 (assay MGI:7861731).
Structure Cell Type Level Pattern Image Note
TS19: mesenchyme derived from neural crest melanoblast Present 6C Ednrb-/-:Aim2-/- PMEL17 , 6C Ednrb-/-:Aim2-/- , 6C Ednrb-/-:Aim2-/- merge
Specimen 6E WT PMEL17: embryonic day 11.5 (more )
Note: Double labeled: green - Pmel; red - Akt1 (assay MGI:7861732).
Structure Cell Type Level Pattern Image Note
TS19: mesenchyme derived from neural crest melanoblast Present 6E WT PMEL17 , 6E WT , 6E WT merge
Specimen 6E Ednrb-/- PMEL17: embryonic day 11.5; Ednrbtm1Myks/Ednrbtm1Myks (more )
Note: Double labeled: green - Pmel; red - Akt1 (assay MGI:7861732).
Structure Cell Type Level Pattern Image Note
TS19: mesenchyme derived from neural crest melanoblast Present 6E Ednrb-/- , 6E Ednrb-/- merge , 6E Ednrb-/- PMEL17
Specimen 6E Ednrb-/-; Aim2-/- PMEL17: embryonic day 11.5; Aim2em2.1Gpt/Aim2em2.1Gpt, Ednrbtm1Myks/Ednrbtm1Myks (more )
Note: Double labeled: green - Pmel; red - Akt1 (assay MGI:7861732).
Structure Level Pattern Image Note
TS19: mesenchyme derived from neural crest Present 6E Ednrb-/-:Aim2-/- PMEL17 , 6E Ednrb-/-:Aim2-/- merge , 6E Ednrb-/-:Aim2-/-
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/25/2025
MGI 6.24 		The Jackson Laboratory