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Gene Expression Data
Assay Details
Assay
Reference: J:175804 Brzezinski JA 4th, et al., Ascl1 expression defines a subpopulation of lineage-restricted progenitors in the mammalian retina. Development. 2011 Aug;138(16):3519-31
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7855853
Gene symbol: Pou4f1
Gene name: POU domain, class 4, transcription factor 1
Antibody: anti-Brn-3 (C-13): sc-6026
Assay notes: The specificity of the antibody used was not detailed; all family members have been annotated.
Results
Specimen 5D: embryonic day 13.5; Ascl1tm1Reed/Ascl1+, Atoh7tm3(cre)Gan/Atoh7+ (more )
Note: Triple labeled: red - Pou4f1 and Pou4f2 and Pou4f3; green - Ascl1 reporter (assay MGI:7855848); white - Atoh7 reporter (assay MGI:7855852).
Structure Cell Type Level Pattern Image Note
TS21: retina ganglion cell layer retinal ganglion cell Present 5D
Specimen 5H: embryonic day 13.5; Ascl1tm1Reed/Ascl1+, Atoh7tm3(cre)Gan/Atoh7+ (more )
Note: Triple labeled: red - Pou4f1 and Pou4f2 and Pou4f3; green - Ascl1 reporter (assay MGI:7855848); white - Atoh7 reporter (assay MGI:7855852).
Structure Cell Type Level Pattern Image Note
TS21: retina ganglion cell layer retinal ganglion cell Present 5H
Specimen 5I: embryonic day 14.5; Ascl1tm1Reed/Ascl1+ (more )
Structure Cell Type Level Pattern Image Note
TS22: retina ganglion cell layer retinal ganglion cell Present 5I
Specimen 5J: embryonic day 14.5; Ascl1tm1Reed/Ascl1tm1Reed (more )
Structure Cell Type Level Pattern Image Note
TS22: retina ganglion cell layer retinal ganglion cell Present 5J Similar as in the control.
Specimen 5K: embryonic day 18.5; Ascl1tm1Reed/Ascl1+ (more )
Structure Cell Type Level Pattern Image Note
TS26: retina ganglion cell layer retinal ganglion cell Present 5K
Specimen 5L: embryonic day 18.5; Ascl1tm1Reed/Ascl1tm1Reed (more )
Structure Cell Type Level Pattern Image Note
TS26: retina ganglion cell layer retinal ganglion cell Present 5L Similar as in the control.
Specimen S3A: embryonic day 13.5 (more )
Structure Cell Type Level Pattern Image Note
TS21: retina ganglion cell layer retinal ganglion cell Present S3A One third (36%) of retinal cells were positive.
Specimen S3C: postnatal day 22 (more )
Note: Double labeled: red - Pou4f1 and Pou4f2 and Pou4f3; green - Calb1 (assay MGI:7855855).
Structure Cell Type Level Pattern Image Note
TS28: retina ganglion cell layer retinal ganglion cell Present S3C 14% of E13.5 birthdated cells adopted retinal ganglion cell fate.
Specimen S7D: embryonic day 18.5; Ascl1tm1Reed/Ascl1+, Atoh7tm3(cre)Gan/Atoh7+ (more )
Note: Triple labeled: red - Pou4f1 and Pou4f2 and Pou4f3; green - Ascl1 reporter (assay MGI:7855848); white - Atoh7 reporter (assay MGI:7855852).
Structure Cell Type Level Pattern Image Note
TS26: retina ganglion cell layer retinal ganglion cell Present S7D
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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