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Gene Expression Data
Assay Details
Assay
Reference: J:175804 Brzezinski JA 4th, et al., Ascl1 expression defines a subpopulation of lineage-restricted progenitors in the mammalian retina. Development. 2011 Aug;138(16):3519-31
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7855848
Gene symbol: Ascl1
Gene name: achaete-scute family bHLH transcription factor 1
Reporter: GFP
Antibody: anti-GFP (ab13970)
Results
Specimen 1A: embryonic day 12.5; Ascl1tm1Reed/Ascl1+ (more )
Note: Double labeled: green - Ascl1 reporter; red - Neurog2 (assay MGI:7855849).
Structure Cell Type Level Pattern Image Note
TS20: retina retinal progenitor cell Present 1A Expressed in nuclei in the progenitor zone of the central retina.
Specimen 1B: embryonic day 13.5; Ascl1tm1Reed/Ascl1+ (more )
Note: Double labeled: green - Ascl1 reporter; red - Neurog2 (assay MGI:7855849).
Structure Level Pattern Image Note
TS21: retina Present 1B Expression had spread towards, but not yet reached, the far periphery.
Specimen 1C/E: embryonic day 14.5; Ascl1tm1Reed/Ascl1+ (more )
Note: Double labeled: green - Ascl1 reporter; red - Sox2 (assay MGI:7855851).
Structure Level Pattern Image Note
TS22: retina Present Regionally restricted 1E , 1C Expression extended to the far peripheral retina and positve nuclei were absent from the ganglion cell layer. The majority coexpressed with Sox2.
TS22: retina ganglion cell layer Absent 1C , 1E
Specimen 1F/I/J/L: embryonic day 14.5; Ascl1tm1Reed/Ascl1+ (more )
Note: Triple labeled: green - Ascl1 reporter; red - Neurog2 (assay MGI:7855849); white - Olig2 (assay MGI:7855850).
Structure Level Pattern Image Note
TS22: retina Present 1F , 1J , 1L , 1I Cells that expressed Ascl1-GFP only; Olig2 only; Neurog2 only; Ascl1-GFP and Olig2; Ascl1-GFP and Neurog2; Olig2 and Neurog2; and all three transcription factors were observed.
Specimen 1M: embryonic day 14.5; Ascl1tm1Reed/Ascl1tm1Reed (more )
Note: Triple labeled: green - Ascl1 reporter; red - Neurog2 (assay MGI:7855849); white - Olig2 (assay MGI:7855850).
Structure Level Pattern Image Note
TS22: retina Present 1M Similar as in the control.
Specimen 5A/C/D: embryonic day 13.5; Ascl1tm1Reed/Ascl1+, Atoh7tm3(cre)Gan/Atoh7+ (more )
Note: Triple labeled: green - Ascl1 reporter; white - Atoh7 reporter (assay MGI:7855852); red - Pou4f1 (assay MGI:7855853) and Pou4f2 (assay MGI:7856524) (assay MGI:7855853) and Pou4f3 (assay MGI:7856525).
Structure Level Pattern Image Note
TS21: retina Present 5A , 5D , 5C The central retinas had many cells that co-expressed Atoh7/Math5-Cre, but none co-expressed Brn3/Pou4f1/2/3.
Specimen 5E/G/H: embryonic day 13.5; Ascl1tm1Reed/Ascl1+, Atoh7tm3(cre)Gan/Atoh7+ (more )
Note: Triple labeled: green - Ascl1 reporter; white - Atoh7 reporter (assay MGI:7855852); red - Pou4f1 (assay MGI:7855853) and Pou4f2 (assay MGI:7856524) (assay MGI:7855853) and Pou4f3 (assay MGI:7856525).
Structure Level Pattern Image Note
TS21: retina Present 5H , 5E , 5G In peripheral retinas, only one cell coexpressed Math5/Atoh7-Cre and Brn3/Pou4f1/2/3.
Specimen S1A/D: embryonic day 18.5; Ascl1tm1Reed/Ascl1+ (more )
Note: Triple labeled: green - Ascl1 reporter; red - Neurog2 (assay MGI:7855849); white - Olig2 (assay MGI:7855850).
Structure Level Pattern Image Note
TS26: retina Present S1D , S1A Cells that expressed Ascl1-GFP only; Olig2 only; Neurog2 only; Ascl1-GFP and Olig2; Ascl1-GFP and Neurog2; Olig2 and Neurog2; and all three transcription factors were observed.
Specimen S1E/H: embryonic day 18.5; Ascl1tm1Reed/Ascl1tm1Reed (more )
Note: Triple labeled: green - Ascl1 reporter; red - Neurog2 (assay MGI:7855849); white - Olig2 (assay MGI:7855850).
Structure Level Pattern Image Note
TS26: retina Present S1E , S1H Mutant animals had each population as in the control, but there were fewer Ascl1-GFP+, Neurog2+ and Olig2+ cells seen.
Specimen S7A/C/D: embryonic day 18.5; Ascl1tm1Reed/Ascl1+, Atoh7tm3(cre)Gan/Atoh7+ (more )
Note: Triple labeled: green - Ascl1 reporter; white - Atoh7 reporter (assay MGI:7855852); red - Pou4f1 (assay MGI:7855853) and Pou4f2 (assay MGI:7856524) (assay MGI:7855853) and Pou4f3 (assay MGI:7856525).
Structure Level Pattern Image Note
TS26: retina Present S7A , S7C , S7D No coexpression with Brn3/Pou4f1/2/3. Many cells co-expressed Atoh7/Math5-Cre.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory