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Gene Expression Data
Assay Details
Assay
Reference: J:355681 Dias Mirandela M, et al., Two-factor authentication underpins the precision of the piRNA pathway. Nature. 2024 Oct;634(8035):979-985
Assay type: In situ reporter (knock in)
MGI Accession ID: MGI:7787142
Gene symbol: Spocd1
Gene name: SPOC domain containing 1
Reporter: HA
Antibody: anti-HA tag
Detection system: Secondary antibody
Results
Specimen 1a PBS: embryonic day 16.5; Spocd1em2Doca/Spocd1+ (more )
Note: Double labeled: red - Spocd1 reporter; green - Piwil4 (assay MGI:7787114).
Structure Level Pattern Image Note
TS24: germ cell of testis Present 1a upper HA , 1a upper merge Accumulated in the nuclei.
Specimen 1b upper: embryonic day 16.5; Piwil4tm3.1Doca/Piwil4+, Spocd1em2Doca/Spocd1+ (more )
Structure Level Pattern Image Note
TS24: germ cell of testis Present 1b upper
Specimen 1b lower: embryonic day 16.5; Piwil4tm3.1Doca/Piwil4tm3.1Doca, Spocd1em2Doca/Spocd1+ (more )
Structure Level Pattern Image Note
TS24: germ cell of testis Present 1b lower Similar as in the control.
Specimen EDF 1a PBS: embryonic day 16.5; Spocd1em2Doca/Spocd1+ (more )
Note: Double labeled: red - Spocd1 reporter; green - Piwil4 (assay MGI:7787114).
Structure Level Pattern Image Note
TS24: germ cell of testis Present EDF1a PBS HA , EDF1a PBS merge
Specimen EDF 1b upper: embryonic day 16.5; Piwil4tm3.1Doca/Piwil4+, Spocd1em2Doca/Spocd1+ (more )
Structure Level Pattern Image Note
TS24: germ cell of testis Present EDF1b upper Accumulated in the nuclei.
Specimen EDF 1b lower: embryonic day 16.5; Piwil4tm3.1Doca/Piwil4tm3.1Doca, Spocd1em2Doca/Spocd1+ (more )
Structure Level Pattern Image Note
TS24: germ cell of testis Present EDF1b lower Similar as in the control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory