Piwil4^tm3.1Doca
Targeted Allele Detail

Summary

• Symbol: Piwil4^tm3.1Doca
• Name: piwi-like RNA-mediated gene silencing 4; targeted mutation 3.1, Donal O'Carroll
• MGI ID: MGI:6468292
• Synonyms: Miwi2^tdTomato, Miwi2^Tom
• Gene: Piwil4 Location: Chr9:14613072-14651968 bp, - strand Genetic Position: Chr9, 4.25 cM
• Alliance: Piwil4^tm3.1Doca page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:243655
• Parent Cell Line: A9 (ES Cell)
• Strain of Origin: (C57BL/6 x 129P2/OlaHsd)F1

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The tdTomato red fluorescent protein gene, preceded by an artificial intron and followed by bovine growth hormone gene poly(A) signal sequence, and an FRT site flanked neomycin resistance gene cassette were inserted into the endogenous ATG start codon. The neo cassette was removed through subsequent flp-mediated recombination, leaving a reporter null allele. (J:243655)

Expression

In Mice Carrying this Mutation:

2 assay results 1 RNA-Seq or microarray experiment(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Piwil4 Mutation: 49 strains or lines available

References

• Original: J:243655 Carrieri C, et al., A transit-amplifying population underpins the efficient regenerative capacity of the testis. J Exp Med. 2017 Jun 05;214(6):1631-1641
• All: 5 reference(s)