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Gene Expression Data
Assay Details
Assay
Reference: J:298691 Wanigasuriya I, et al., Smchd1 is a maternal effect gene required for genomic imprinting. Elife. 2020 Nov 13;9:e55529
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7785332
Gene symbol: Smchd1
Gene name: SMC hinge domain containing 1
Antibody: Anti-Smchd1 #8 (Clone 2B8)
Detection system: Secondary antibody coupled to DyLight 550
Assay notes: Two antibodies were described: MGI:7785316 and MGI:7785314. The antibody used in this assay was not specified, so either was possible.
Results
Specimen 1b Primordial Control: postnatal week 6-14 (more )
Note: Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of primordial follicle Present 1b top row Primordial
Specimen 1b Primordial MMTV-Cre: postnatal week 6-14; Smchd1tm1.1Maar/Smchd1tm1.2Maar, Tg(MMTV-cre)1Mam/0 (more )
Note: Conditional mutant. Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of primordial follicle Present 1b middle row Primordial
Specimen 1b Primordial Zp3-Cre: postnatal week 6-14; Smchd1tm1.1Maar/Smchd1tm1.1Maar, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of primordial follicle Present 1b bottom row Primordial
Specimen 1b Primary Control: postnatal week 6-14 (more )
Note: Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of primary follicle Present 1b top row Primary
Specimen 1b Primary MMTV-Cre: postnatal week 6-14; Smchd1tm1.1Maar/Smchd1tm1.2Maar, Tg(MMTV-cre)1Mam/0 (more )
Note: Conditional mutant. Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of primary follicle Present 1b middle row Primary Expression was not affected in primary follicles in the MMTV-Cre conditional mutant.
Specimen 1b Primary Zp3-Cre: postnatal week 6-14; Smchd1tm1.1Maar/Smchd1tm1.1Maar, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of primary follicle Weak 1b bottom row Primary Positive staining dropped to 10% in the Zp3-Cre conditional mutant in the primary follicle.
Specimen 1b Early secondary Control: postnatal week 6-14 (more )
Note: Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of secondary follicle Present 1b top row Early secondary
Specimen 1b Early secondary MMTV-Cre: postnatal week 6-14; Smchd1tm1.1Maar/Smchd1tm1.2Maar, Tg(MMTV-cre)1Mam/0 (more )
Note: Conditional mutant. Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of secondary follicle Present 1b middle row Early secondary Positive staining dropped to 70% in the MMTV-Cre conditional mutant in the early secondary follicle.
Specimen 1b Early secondary Zp3-Cre: postnatal week 6-14; Smchd1tm1.1Maar/Smchd1tm1.1Maar, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of secondary follicle Absent 1b bottom row Early secondary Staining was lost in the Zp3-Cre conditional mutant early secondary follicle.
Specimen 1b Late secondary Control: postnatal week 6-14 (more )
Note: Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of secondary follicle Present 1b top row Late secondary
Specimen 1b Late secondary MMTV-Cre: postnatal week 6-14; Smchd1tm1.1Maar/Smchd1tm1.2Maar, Tg(MMTV-cre)1Mam/0 (more )
Note: Conditional mutant. Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of secondary follicle Present 1b middle row Late secondary Positive staining dropped to 30% in the MMTV-Cre conditional mutant in the late secondary follicle.
Specimen 1b Late secondary Zp3-Cre: postnatal week 6-14; Smchd1tm1.1Maar/Smchd1tm1.1Maar, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of secondary follicle Absent 1b bottom row Late secondary Staining was lost in the Zp3-Cre conditional mutant late secondary follicle.
Specimen 1b Antral Control: postnatal week 6-14 (more )
Note: Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of antral follicle Present 1b top row Antral
Specimen 1b Antral MMTV-Cre: postnatal week 6-14; Smchd1tm1.1Maar/Smchd1tm1.2Maar, Tg(MMTV-cre)1Mam/0 (more )
Note: Conditional mutant. Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of antral follicle Absent 1b middle row Antral Staining was lost in the MMTV-Cre conditional mutant antral follicle.
Specimen 1b Antral Zp3-Cre: postnatal week 6-14; Smchd1tm1.1Maar/Smchd1tm1.1Maar, Tg(Zp3-cre)3Mrt/0 (more )
Note: Conditional mutant. Double labeled: magenta - Smchd1; yellow - Kit (assay MGI:7785339).
Structure Level Pattern Image Note
TS28: oocyte of antral follicle Absent 1b bottom row Antral Staining was lost in the Zp3-Cre conditional mutant antral follicle.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory