About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:66266 Lewandoski M, et al., Fgf8 signalling from the AER is essential for normal limb development. Nat Genet. 2000 Dec;26(4):460-3
Assay type: RNA in situ
MGI Accession ID: MGI:7720845
Gene symbol: Shh
Gene name: sonic hedgehog
Probe: Shh probe
Probe preparation: RNA
Results
Specimen 2a: embryonic day 9.5; Fgf8tm1.3Mrt/Fgf8+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant. 27-28 somites
Structure Level Pattern Image Note
TS15: forelimb bud Trace 2a
Specimen 2b: embryonic day 9.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant. 27-28 somites
Structure Level Pattern Image Note
TS15: forelimb bud Absent 2b Expression delayed by at least two somite stages.
Specimen 2c: embryonic day 10.0; Fgf8tm1.3Mrt/Fgf8+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant. 29-30 somites
Structure Level Pattern Image Note
TS16: forelimb bud Present 2c
Specimen 2d: embryonic day 10.0; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant. 29-30 somites
Structure Level Pattern Image Note
TS16: forelimb bud Present 2d
Specimen 2e: embryonic day 11.5; Fgf8tm1.3Mrt/Fgf8+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: forelimb bud Present 2e
Specimen 2f: embryonic day 11.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: forelimb bud Present 2f
Specimen 2g: embryonic day 10.0; Fgf8tm1.3Mrt/Fgf8+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant. 33-34 somites
Structure Level Pattern Image Note
TS16: hindlimb bud Present 2g
Specimen 2h: embryonic day 10.0; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant. 33-34 somites
Structure Level Pattern Image Note
TS16: hindlimb bud Absent 2h
Specimen 2i: embryonic day 10.5; Fgf8tm1.3Mrt/Fgf8+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant. 35-36 somites
Structure Level Pattern Image Note
TS17: hindlimb bud Present 2i
Specimen 2j: embryonic day 10.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant. 35-36 somites
Structure Level Pattern Image Note
TS17: forelimb bud Trace 2j
Specimen 2k: embryonic day 11.5; Fgf8tm1.3Mrt/Fgf8+, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: hindlimb bud Present 2k
Specimen 2l: embryonic day 11.5; Fgf8tm1.3Mrt/Fgf8tm1.4Mrt, Tg(Msx2-cre)5Rem/0 (more )
Note: Conditional mutant.
Structure Level Pattern Image Note
TS19: hindlimb bud Present 2l Expression domain is small but in proportion to size of limb bud.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
01/28/2026
MGI 6.24 		The Jackson Laboratory