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Gene Expression Data
Assay Details
Assay
Reference: J:215404 Hering TM, et al., Changes in type II procollagen isoform expression during chondrogenesis by disruption of an alternative 5' splice site within Col2a1 exon 2. Matrix Biol. 2014 Jun;36:51-63
Assay type: Immunohistochemistry
MGI Accession ID: MGI:7716947
Gene symbol: Col2a1
Gene name: collagen, type II, alpha 1
Antibody: Anti-type II collagen
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 6A: postnatal day 7 (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: tibia epiphysis Present Widespread 6A Abundant expression of the triple-helical domain of type II collagen was detected in the cartilage.
Specimen 6B: postnatal day 7; Col2a1tm2.1Mcali/Col2a1+ (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: tibia epiphysis Present Widespread 6B In the ki/+ tissue, expression of the triple-helical domain co-localized with abundant isoform IIA expression throughout the cartilage.
Specimen 6C: postnatal day 7; Col2a1tm2.1Mcali/Col2a1tm2.1Mcali (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: tibia epiphysis Present Widespread 6C In the ki/ki tissue, expression of the triple-helical domain co-localized with abundant isoform IIA expression throughout the cartilage.
Specimen 6D: postnatal day 14 (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: long bone epiphysis Present 6D
Specimen 6E: postnatal day 14; Col2a1tm2.1Mcali/Col2a1+ (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: long bone epiphysis Present 6E
Specimen 6F: postnatal day 14; Col2a1tm2.1Mcali/Col2a1tm2.1Mcali (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: long bone epiphysis Present 6F
Specimen 6G: postnatal day 28 (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: long bone epiphysis Present 6G
Specimen 6H: postnatal day 28; Col2a1tm2.1Mcali/Col2a1+ (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: long bone epiphysis Present 6H
Specimen 6I: postnatal day 28; Col2a1tm2.1Mcali/Col2a1tm2.1Mcali (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: long bone epiphysis Present 6I
Specimen 7A: postnatal day 56 (more )
Note: Section was obtained from the anterior region of the joint. Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: long bone epiphysis Present 7A
Specimen 7B: postnatal day 56; Col2a1tm2.1Mcali/Col2a1+ (more )
Note: Section was obtained from the anterior region of the joint. Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: long bone epiphysis Present 7B
Specimen 7C: postnatal day 56; Col2a1tm2.1Mcali/Col2a1tm2.1Mcali (more )
Note: Section was obtained from a region near the middle of the joint. Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: long bone epiphysis Present 7C
Specimen 7D: postnatal day 56 (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: long bone epiphysis Present 7D
Specimen 7E: postnatal day 56; Col2a1tm2.1Mcali/Col2a1+ (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: long bone epiphysis Present 7E
Specimen 7F: postnatal day 56; Col2a1tm2.1Mcali/Col2a1tm2.1Mcali (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: long bone epiphysis Present 7F
Specimen 8A: postnatal day 56; Col2a1tm2.1Mcali/Col2a1+ (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: articular cartilage Present 8A
Specimen 8B: postnatal day 56; Col2a1tm2.1Mcali/Col2a1+ (more )
Note: Double labeled: red - Col2a1; green - Col11a1 (assay MGI:7716960).
Structure Level Pattern Image Note
TS28: articular cartilage Present 8B
Specimen 8C: postnatal day 56; Col2a1tm2.1Mcali/Col2a1+ (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: epiphyseal plate Present 8C
Specimen 8D: postnatal day 56; Col2a1tm2.1Mcali/Col2a1+ (more )
Note: Double labeled: red - Col2a1; green - Col11a1 (assay MGI:7716960).
Structure Level Pattern Image Note
TS28: epiphyseal plate Present 8D
Specimen 8E: postnatal day 56 (more )
Note: Double labeled: red - Col2a1 protein; green - Col2a1 protein (assay MGI:7716915).
Structure Level Pattern Image Note
TS28: epiphyseal plate Present 8E
Specimen 8F: postnatal day 56 (more )
Note: Double labeled: red - Col2a1; green - Col11a1 (assay MGI:7716960).
Structure Level Pattern Image Note
TS28: epiphyseal plate Present 8F
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory