Antibody:	Anti-Type IIA procollagen
Detection system:	Secondary antibody coupled to Alexa Fluor 488

Structure	Level	Pattern	Image	Note
TS20: hindlimb digit mesenchyme	Present	Regionally restricted	5A	Expression was detected in the cartilage of the developing digits at E12.5.

Structure	Level	Pattern	Image	Note
TS20: hindlimb digit mesenchyme	Present	Regionally restricted	5B	No apparent differences in staining pattern was observed in cartilaginous regions of E12.5 developing limb buds compared with wild type.

Structure	Level	Pattern	Image	Note
TS20: hindlimb digit mesenchyme	Present	Regionally restricted	5C	No apparent differences in staining pattern was observed in cartilaginous regions of E12.5 developing limb buds compared with wild type.

Structure	Level	Pattern	Image	Note
TS24: hindlimb cartilage condensation	Present	Regionally restricted	5D	At E16.5 abundant IIA staining was seen at the most proximal end of wild type developing tibiae where higher levels of chondroprogenitor cells are present.
TS24: tibia	Present	Regionally restricted	5D	At E16.5 abundant IIA staining was seen at the most proximal end of wild type developing tibiae where higher levels of chondroprogenitor cells are present.
TS24: epiphyseal plate proliferative zone	Present	Non-Uniform	5D	A "lacy" localization pattern was observed in the proliferative zone.
TS24: epiphyseal plate hypertrophic zone	Trace		5D	Barely detectable levels were seen in the hypertrophic zone.

Structure	Level	Pattern	Image	Note
TS24: tibia	Present	Uniform	5E	Increased staining pattern was observed in the ki/+ tibia; expression was seen throughout the entire tibia.

Structure	Level	Pattern	Image	Note
TS24: tibia	Present	Uniform	5F	Increased staining pattern was observed in the ki/ki tibia; expression was seen throughout the entire tibia.

Structure	Level	Pattern	Image	Note
TS27: tibia	Present	Regionally restricted	5G	At P0 abundant IIA staining was seen at the most proximal end of wild type developing tibiae where higher levels of chondroprogenitor cells are present.
TS27: epiphyseal plate proliferative zone	Present	Non-Uniform	5G	A "lacy" localization pattern was observed in the proliferative zone.
TS27: epiphyseal plate hypertrophic zone	Trace		5G	Barely detectable levels were seen in the hypertrophic zone.

Structure	Level	Pattern	Image	Note
TS27: tibia	Present	Uniform	5H	Increased staining pattern was observed in the ki/+ tibia; expression was seen throughout the entire tibia.

Structure	Level	Pattern	Image	Note
TS27: tibia	Present	Uniform	5I	Increased staining pattern was observed in the ki/ki tibia; expression was seen throughout the entire tibia.

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Present	Regionally restricted	6A	The majority of isoform IIA expression remaining was concentrated in the superficial zone of the articular cartilage.

Structure	Level	Pattern	Image	Note
TS28: tibia epiphysis	Present	Widespread	6B	The ki/+ tissue shows abundant isoform IIA expression throughout cartilage of the developing tibia that co-localizes with the triple-helical domain of type II collagen. A loss of isoform IIA staining was noticeable at the most proximal region of the developing ki/+ tibia.

Structure	Level	Pattern	Image	Note
TS28: tibia epiphysis	Present	Widespread	6C	The ki/ki tissue shows abundant isoform IIA expression throughout cartilage of the developing tibia that co-localizes with the triple-helical domain of type II collagen.

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Present		6D

Structure	Level	Pattern	Image	Note
TS28: tibia epiphysis	Weak	Regionally restricted	6E	There was an apparent reduction of isoform IIA staining in the extracellular matrix of ki/+ tissue that will become mature articular cartilage.
TS28: epiphyseal plate	Present	Regionally restricted	6E	There was still abundant IIA staining in the highly cellular growth plate cartilage region below the secondary ossification center.

Structure	Level	Pattern	Image	Note
TS28: long bone epiphysis	Weak	Regionally restricted	6F	There was an apparent reduction of isoform IIA staining in the extracellular matrix of ki/ki tissue that will become mature articular cartilage.
TS28: epiphyseal plate	Present	Regionally restricted	6F	There was still abundant IIA staining in the highly cellular growth plate cartilage region below the secondary ossification center.

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Present		6G	Expression was detected in the superficial zone of the articular cartilage.

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Present		6H	The superficial zone of the articular cartilage was stained in the ki/+ tissue. Staining was also detected in the pericellular domain surrounding a number of chondrocytes located throughout the entire depth of the articular cartilage.
TS28: epiphyseal plate proliferative zone	Present	Non-Uniform	6H	Loss of IIA staining can be seen in the extracellular matrix of the upper proliferative zone of growth plate cartilage, but expression is still persistent in the pericellular region of growth plate cartilage cells in the lower proliferative region.
TS28: epiphyseal plate hypertrophic zone	Present	Regionally restricted	6H	Expression is still persistent in the pericellular region of growth plate cartilage cells in the prehypertrophic and hypertrophic regions.

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Present	Regionally restricted	6I	The superficial zone of the articular cartilage was stained in the ki/ki tissue. Staining was also detected in the pericellular domain surrounding a number of chondrocytes located throughout the entire depth of the articular cartilage.
TS28: epiphyseal plate proliferative zone	Present	Non-Uniform	6I	Loss of IIA staining can be seen in the extracellular matrix of the upper proliferative zone of growth plate cartilage, but expression is still persistent in the pericellular region of growth plate cartilage cells in the lower proliferative region.
TS28: epiphyseal plate hypertrophic zone	Present	Regionally restricted	6I	Expression is still persistent in the pericellular region of growth plate cartilage cells in the prehypertrophic and hypertrophic regions.

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Present	Regionally restricted	7A	Expression was detected in the superficial zone of the articular cartilage.

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Present	Regionally restricted	7B	Isoform IIA expression persisted in ki/+ articular cartilage at both the superficial zone and in the pericellular domain of deeper zone chondrocytes.

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Present	Regionally restricted	7C	Isoform IIA expression persisted in ki/ki articular cartilage at both the superficial zone and in the pericellular domain of deeper zone chondrocytes.

Structure	Level	Pattern	Image	Note
TS28: epiphyseal plate	Absent		7D	No IIA staining was observed in the growth plate of P56 wild type mice.

Structure	Level	Pattern	Image	Note
TS28: epiphyseal plate	Present	Regionally restricted	7E	Persistence of IIA staining was found in the growth plate of region of ki/+ tissue, especially in the pericellular region of the extracellular matrix.

Structure	Level	Pattern	Image	Note
TS28: epiphyseal plate	Present	Regionally restricted	7F	Persistence of IIA staining was found in the growth plate of region of ki/ki tissue, especially in the pericellular region of the extracellular matrix.

Structure	Level	Pattern	Image	Note
TS28: articular cartilage	Present	Regionally restricted	8A	Isoform IIA expression persisted in ki/+ articular cartilage at both the superficial zone and in the pericellular domain of deeper zone chondrocytes.

Structure	Level	Pattern	Image	Note
TS28: epiphyseal plate	Present		8C	IIA staining was found in the growth plate of region of ki/+ tissue, especially in the pericellular region of the extracellular matrix.

Structure	Level	Pattern	Image	Note
TS28: epiphyseal plate	Absent		8E	No IIA staining was observed in the growth plate of P56 wild type mice.